Identification Of Process Conditions Influencing Protein Aggregation In Chinese Hamster Ovary Cell Culture

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Criteria for scale-up and scale-down of bioreactors for

by B Neunstoecklin 2014 Cited by 1 operating range for parameters affecting the cell culture process. The first was derived from a Chinese hamster ovary. (CHO) host cell line and the 

PDF (7 MB) - ACS Publications - American Chemical Society

by S Pérez-Rodriguez 2021 protein production in Chinese hamster ovary (CHO) cells have identification of targets related to various cellular processes.

Trends in Upstream and Downstream Process - MDPI

by P Gronemeyer 2014 Cited by 251 enormously in cell culture processes [1,2,5 8] by developing recombinant proteins are Chinese Hamster Ovary (CHO) cells [5,8,9,43,45].

Cell culture processes for biopharmaceutical - JSTOR

by M Gadgil 2017 Cited by 4 Medium and process conditions are optimized for the identified cell line to in and include Chinese hamster ovary (CHO) cells, baby.

Multiplex secretome engineering enhances - bioRxiv

24 May 2019 create a clean Chinese hamster ovary (CHO) cell by disrupting multiple genes to eliminate HCPs. Using a model of CHO cell protein 

The impact of process temperature on mammalian cell lines

the Chinese hamster ovary (CHO) cell [2] lation initiation factors [17], culture process develop- ment [18] and the use of subphysiological temperature.13 pages

Overexpression of microRNAs enhances recombinant protein

by WP Loh 2014 Cited by 45 and low- monoclonal antibody-producing Chinese hamster ovary (CHO) cell clones using next- ture processes and thus recombinant protein production.


Process Glucose Control to Influence Biopharmaceutical Protein Product Background and novelty: Chinese hamster ovary (CHO) cell culture has been 

Integrating Upstream and Downstream Process Development

by LJ Wilson 2020 Effects of upstream culture conditions on mAb-associated post-protein A HCP of making these modifications such as Chinese hamster ovary (CHO) cells are.227 pages

Systematic development of temperature shift strategies for

by J Xu 2019 Cited by 21 processes that use Chinese hamster ovary (CHO) cells. metabolites, protein titer and quality depends on cell line, product, 

The effect of potential large-scale bioreactor environmental

by WH Scott Within the cell, process conditions can cause aggregation by altering protein Chinese hamster ovary (CHO) cells are widely used to produce recombinant.

Screening Naturally Occurring Phenolic Antioxidants - MDPI

3 Jun 2019 diet for their effect on Chinese hamster ovary cells and the F. Identification of process conditions influencing protein aggregation.

Chapter 1 Cell Culture Process Optimization - OPUS Würzburg

by D Brühlmann 2017 Cited by 70 the focus in mammalian cell-culture process development. several rodent-derived cell lines Chinese hamster ovary (CHO) cells, 

Improving culture performance and antibody - UQ eSpace

by M Buchsteiner CHO Cell Culture Processes by Reducing the Warburg Effect. Maria Buchsteiner Keywords: Warburg Effect, Chinese Hamster Ovary cells, Lactate, Pyruvate.

Cell-culture growth conditions resulting in the oxidation of a

by K Siddiquee 2019 Cited by 2 To ensure the quality of the target molecule in the cell-culture process, small-scale Chinese Hamster Ovary cells expressing the recombinant.

parallels between microbial natural products and cell culture

by AA Bandyopadhyay 2017 Cited by 17 cell line development process for recombinant protein manufacturing. d Next-gen cell line development produced using Chinese hamster ovary (CHO) cells.

Conference Program - ECI Digital Archives

13 May 2016 Kathy Chan, Conference Program in Cell Culture Engineering XV , A community genome-scale model of Chinese hamster ovary cell.

The impact of cell culture environment on CHO cell behaviour

by CH Goey Cited by 2 process indicators of CHO cell cultures and post-Protein A purification (mAb Figure 1.14 Silver-stained 2-DE pattern of Chinese hamster ovary cellular  279 pages


by EE Russell 2015 identify factors that support protein aggregation. Studies were performed using three Chinese hamster ovary (CHO) cell lines. Two were clonal.


by P Tang 2018 In this review, we examined key factors for upstream process Keywords: Chinese hamster ovary (CHO), kinetic modeling, cell culture, therapeutic protein.

Understanding the Transcriptional Landscape of Chinese

by YK Gowtham 2016 Cited by 1 Chinese hamster ovary (CHO) cells have been used for over 25 process, we adapted a parental cell line, CHO DHFR-, and a recombinant cell line, rCHO.

Fundamental Techniques in Cell Culture - Sigma-Aldrich

nitrogen store, the identity and purity of such a cell line should be questioned in the absence of a well recorded Chinese Hamster Ovary. Fibroblast.

Process Development -

identified cell line that produces the product and harvest of the product. Chinese Hamster Ovary cells (CHO) and Non-Immunoglobulin Secreting mouse 

N-Glycosylation optimization of recombinant - DTU Orbit

by Y Fan Cited by 3 glycosylation engineering in Chinese hamster ovary (CHO) cells. the knowledge of CHO cell culture technology, upstream process engineering, metabolic.

Investigation of the interactions of critical scale-up parameters

by M Brunner Cited by 52 Keywords CHO cell culture 4 Process parameter 4 Scale- Chinese hamster ovary (CHO) cells are the most commonly used.

An 'omics approach towards CHO cell engineering - CiteSeerX

by P Datta 2013 Cited by 140 Identification of cell culture conditions to control protein aggregation of IgG fusion proteins expressed in Chinese hamster ovary cells. Process.

Literature Review: CHO versus HEK Cell - Maxcyte

by K Steger changes to cell culture conditions ranging from culture stirring speed to nutrient availability can impact and SP2/0) cells, Chinese hamster ovary (CHO).

Impact of Media and Antifoam Selection on - OSTI.GOV

by SR Velugula-Yellela 2017 Cited by 25 Monoclonal antibody production in commercial scale cell culture bioprocessing protein production processes utilize Chinese Hamster Ovary.

Identification of process parameters influencing product

by AJ Paul 2015 allows quantification of mAb aggregate formation directly in cell culture samples of Chinese hamster ovary. (CHO) cells replacing falsifying 

a review of the causes, implications, and potential alleviation

by H Dahodwala 2019 Cited by 28 Chinese hamster ovary (CHO) cell-based bioproduction of process, a stable cell line can be defined as a homogenous cell population that has the 

Genome-wide analysis of recombinant protein - Abstracts

10 Jul 2013 tedious process of mammalian cell line development, Chinese hamster ovary (CHO) cells are currently the workhorses of the 

Recombinant Protein Production Utilising a - UNSWorks

(hGH), produced by a Chinese hamster ovary (CHO) cell line utilising the pNK system at various fermentation scales and modes. As process development for CHO.

Protein Aggregation during Bioprocessing of - OPARU

by A Paul 2017 Cited by 1 influence on protein aggregation in cell cultures of Chinese hamster ovary (CHO) production cell lines. After identification of the most 

PhD thesis - White Rose eTheses Online

48000 copies by YB Johari 2015 Keywords: Chinese hamster ovary cells; transient gene expression; difficult-to-express protein; aggregation; unfolded protein response;  213 pages

Investigating Media Supplements to Enhance Chinese

by B Capella Roca 2020 ammonium citrate was identified and a novel strategy to circumvent this inhibition was Fc-fusion protein in Chinese hamster ovary cell culture.

Stress Factors in mAb Drug Substance Production Processes

by TK Das 2020 Cited by 12 Chinese hamster ovary (CHO) cell culture technology is one of the ucts of the protein.2-4 Protein aggregates of every size, ranging from.

Multi-omics profiling of a CHO cell culture system - Authorea

by A Lee Chinese hamster ovary (CHO) cells are one of the main host systems used Identification of process conditions influencing protein aggre-.

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Identification of cell culture conditions to con- trol protein aggregation of IgG fusion proteins expressed in Chinese hamster ovary cells. Process Biochem 

Erythropoietin provides a molecular model for protein burden

by R Maldonado during'batch'culture'of'recombinant'Chinese'Hamster'Ovary'cells' can contaminate cell culture processes affecting the quality and yield of recombinant.

Characterization of Chinese Hamster Ovary Cell Performance

by J Camire However, carboxylic acids have been shown to promote an apoptotic response in CHO cell culture. Pentanoic acid is a carboxylic acid that has demonstrated lower 

Identification of process conditions influencing protein

by AJ Paul 2018 Cited by 14 DOI: 10.1002/bit.26534. ARTICLE. Identification of process conditions influencing protein aggregation in Chinese hamster ovary cell culture.

PhD thesis Maja Stressmann 2 - UWSpace - University of

by M Stressmann 2008 Cited by 2 operated at constant permeate flux using bovine serum albumin (BSA) solutions or Chinese hamster ovary (CHO) cell culture broths as feed solutions.

Development of mathematical modelling for the glycosylation

Key words: Chinese hamster ovary cells, glycosylation, IgG, mathematical modelling, 1.3.3 Influence of cultivation factors on the glycosylation 102 pages

Perfusion reduces bispecific antibody aggregation via - Nature

by P Sinharoy 2020 Cited by 1 mechanisms that influence BisAb aggregate formation in the ER. The process conditions for both CHO cell culture processes are outlined in Table 1.

Correlating composition and functionality of soy protein

by AJ Gupta Cited by 1 Chinese hamster ovary (CHO) cells are often used in cell cultures to produce biopharma- Factors affecting composition of hydrolysates.


by S Sandadi 2009 Cited by 1 Consequently, an understanding of the how process conditions affect cell growth and antibody production may lead to improved process performance. Four types of 

Technical application notes on cell culture and - Eppendorf

Intelligent Control of Chinese Hamster Ovary (CHO) CellCulture Using To mimick a typical protein production process, the process.

View Open Manuscript -

by D Radhakrishnan 2018 Cited by 7 Paul AJ, Handrick R, Ebert S, Hesse F: Identification of process conditions influencing protein aggregation in Chinese hamster ovary cell culture.

Secretory leakage of IgG1 aggregates from recombinant

antibodies during the culture of Chinese hamster ovary (CHO) cells to determine how explore antibody aggregation in the CHO cell culture process.6 pages

Development of tools for monitoring and controlling cell

depending on two cell culture conditions: the pH and the lactate Chinese Hamster Ovary (CHO) and Human Embrionik Kidney (HEK293) cell lines (Estes.