Comparison Of Modification Sites In Glycated Crystallin In Vitro And In Vivo

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Comparison of modification sites in glycated crystallin in

K166 in the B chain of α-crystallin as major glycation sites during the in vitro reaction. We found also two in vivo glycated lysine residues: K92 in the B chain and K166 in the A chain, which are known as locations for Amadori products. These modification sites were confirmed by the LC-MS experiment using two synthetic standards. This study