A Stable Isotope Technique For Investigating Lactate Metabolism In Humans

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Decreased insulin action in skeletal muscle from patients

Measurement of whole body metabolism. Whole body glu-cose metabolism was evaluated during the experiment by means of a stable isotope technique (primed infusion of [6,6-2H 2]glucose) and indirect calorimetry and from the infusion rate of unlabeled glucose. From the enrichments of labeled glucose in deproteinized plasma samples (33), the rates of

1 2Author Manuscript NIH Public Access Gülin Öz1 NMR Biomed1

On the other hand, tracer studies can be performed using stable isotopes. The metabolism of stable isotopes can be followed non-invasively using NMR, although administration of the tracer requires a higher isotopic enrichment, for 13C, typically above 50% in the precursor pool.

Altered skeletal muscle fatty acid handling is associated

the postprandial state in healthy lean humans, even though VLDL particles were abundantly present after the meal [11]. Up to now, most studies investigating combined VLDL-and chylomicron-TAG metabolism have been performed in healthy,leanhumans[17,18].Bickertonetal[11]haveshown elevated postprandial plasma VLDL- and chylomicron-TAG

Review Article Localized in vivo C NMR spectroscopy of the brain

metabolism non-invasively. When using radiotracers, label in different metabolic pools cannot be distin-guished, which has led to the use of non-metabolizable analogs, such as deoxy-glucose. On the other hand, tracer studies can be performed using stable isotopes. The metabolism of stable isotopes can be

Metabolomics and Isotope Tracing - Cell

achieved by investigating both. Unlike metabolites, fluxes are not physical entities that can be measured in a mass spectrometer. They can be inferred, how-ever, through use of isotope tracers. Classical radioactive tracer studies laid the foundations for modern understanding of meta-bolism. Today, similar studies can be performed using MS or

ORIGINAL ARTICLE Intramuscular Lipid Metabolism in the

M., when an antecubital vein in one arm was cannulated for isotope infusion and a retrograde dorsal hand vein in the contralateral side was catheterized for blood sampling via the heated hand technique. At 7:30 A.M., blood and breath samples were taken for background isotope enrichment. Starting at 8 A.M., a primed (4.5 mg/kg) constant (0.03 mg

Myocardial Substrate Utilization during Exercise in Humans

Theisotopic lactate extraction ratio (%) for ['4Cllactate wascalcu-latedas([A] Xspactlactate inartery -[CS] Xspactlactate inCS)/([A] Xsp act lactate in artery) X 100. The myocardial isotopic lactate extraction (.umol/ml) was deter-minedfromeitherthe [U-'3C]lactate or[14C]lactate extraction ratio as [A] X isotopic lactate extraction ratio

University of Groningen In vivo estimation of gluconeogenesis

radioactive or stable isotope labeling of individual substrates have been employed to determine the proportion of glucose release attributable to gluconeogenesis. About 15 years ago three approaches using compounds labeled with stable isotopes with the potential to accurately estimate total gluconeogenesis were published (11-15). However,

University of Groningen In vivo estimation of gluconeogenesis

isotopomer distribution analysis during the infusion of uniformly 13C labeled stable isotope tracers of glucose/ glycerol or on the deuterium labeling in glucose carbon after the ingestion of deuterium oxide. The approach developed by Landau et al.(15) is widely considered as the golden standard to estimate gluconeogenesis.