Tubulin And Microtubules In The Early Development Of The Axolotl And Other Amphibia

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microtubules, apparently by interacting directly with tubulin, as well as inducing changes in the expression of genes that are implicated in tissue remodelling and wound healing. The second is the conditional expression of the homeobox gene Msx1 in mouse myotubes (Odelberg et al. 2000). Msx1 has been studied as an antagonist of myogenic and

Amer. Zool., 18:237-251 (1978).

Tubulin and Microtubules in the Early Development of the Axolotl and Other Amphibia Elizabeth C. Raff and Rudolf A. Raff Program in Molecular and Cell Biology and Department of Biology, Indiana University, Bloomington, Indiana 47401 synopsis. Unfertilized eggs of the axolotl, Ambystoma mexicanum, contain a pool of soluble tubulin accumulated

doi: 10.1007/978-1-4939-9009-2 2

immunofluorescence (IF) protocols for imaging microtubule assemblies in early amphibian development. We developed these protocols to elucidate how the cell division machinery adapts to drastic changes in embryonic cell sizes. We describe how to image mitotic spindles, microtubule asters, chromosomes, and

Comparative expression analysis of pfdn6a and tcp1a during

On the other hand, in mouse, tcp1 is expressed in post-implantation embryos and the amount of its mRNA decreases during development (from 7.5 to 17.5 day dpc) (Kubota et al., 1992). Differences between amphib-ians and mouse in early tcp1 expression may be ascribed to the possible lack of a maternal transcript in the mouse embryo, which

Comparative expression analysis of pfdn6a and tcp1α during

transcripts of pfnd6a in the animal hemisphere at early blastula stage. During gastrulation, pfdn6a is expressed in the involuting mesoderm and subsequently in the anterior and dorsal neural plate.