Demonstration Of The Lyme Disease Spirochete By A Modified Dieterle Stain Method

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Histopathologic Patterns of Erythema Migrans and Borrelial

spirochete is located in a subepidermal papilla. Modified Steiner method, X960. gold-silver staining. 20-23 Spirochetes stain poorly with monoclonal antibodies in skin biopsies that are fixed in formalin and embedded in paraffin. Polyclonal antibod- ies can be used with good results. The microorganisms

846 Concise Communications

12. Barbour AG Isolation and cultivation of Lyme disease spiro-chetes. Yale J Biol Med 1984;57:521-5 13. Duray PH, Kusnitz A, Ryan J. Demonstration of the Lyme disease spirochete by a modified Dieterle stain method. Laboratory Medicine 1985;11:685-7 14. Craft JE, Grodzicki RL, Steere AC. Antibody response in Lyme disease: evaluation of

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Dieterle stain (2); Churukian-Schenk method (35) All these techniques are based on silver impregnation of spirochetes and need a developer for reducing the silver to a visible metallic form. Warthin-Starry (WS) method, as modified by Luna (9), has been chosen in our Laboratory as a routine method for the demonstration of spirochetes in tissue