Optical Interrogation Of Neural Circuits In Caenorhabditis Elegans

Below is result for Optical Interrogation Of Neural Circuits In Caenorhabditis Elegans in PDF format. You can download or read online all document for free, but please respect copyrighted ebooks. This site does not host PDF files, all document are the property of their respective owners.

382a Monday, February 17, 2014

analysis of neural function and behavior. In the nematode Caenorhabditis elegans, a commonly used model organism for studying neural circuits, optogenetic stimulation of several neuromuscular networks has induced iden-tical behavioral responses to those resulting from mechanical stimulation.

OPTOGENETIC MANIPULATION OF FREELY MOVING C. ELEGANS IN AN

end, optogenetics has been accelerating progress by manipulating the neural system of C. elegans [1-5]. A majority of research designs customized optical control systems and illuminates freely moving transgenic C. elegans to evoke or inhibit certain neurons for the control of neurons and muscles [1], particular circuits [2], motor neurons [3], and

��Dugue, G. P., Akemann, W. & Knopfel, T. A comprehensive

A circuit for navigation in Caenorhabditis elegans. Proc. Natl Acad. Sci. USA 102, 3184 3191 (2005). ��Chalasani, S. H. et al. Dissecting a circuit for olfactory behaviour in Caenorhabditis elegans. Nature 450, 63 70 (2007). ��Chalasani, S. H. et al. Dissecting a circuit for olfactory behaviour in Caenorhabditis elegans.

Optogenetic approaches for dissecting neuromodulation and

in Caenorhabditis elegans muscle cells, demon-strating channel-based activity comparable to photo-stimulation from optogenetics [18 ]. Opto-XRs GPCR signaling in neural circuits may also be modulated though intracellular signaling that may not be dependent solely on generic Ga subunit coupling dynamics. There-fore, meric

Microfluidics for Electrophysiology, Imaging, and Behavioral

Jan 31, 2018 for chemical, optical, and electrical interrogation of C. elegans and Drosophila on microfluidic platforms. This confluence of technologies has revealed how many behaviors can be implemented by neural circuits,20 24 however, C. elegans and D. melanogaster may not be the best suited to study neural circuit repair and remodeling.

Novel Technological Advances in Functional Connectomics in C

which allows for interrogation of both local and global neural circuits, leading to di erent behaviors. Combining these approaches will shed light into how neural networks process sensory information to generate the appropriate behavioral output, providing a complete understanding of the worm nervous system.

Untwisting the Caenorhabditis elegans embryo

to catalog C. elegans neurodevelopment, allowing interrogation of developmental events in previously inaccessible periods of embryogenesis. DOI: 10.7554/eLife.10070.001 Introduction Understanding how complex neural circuits and entire nervous systems form is one of the fundamen-tal goals of neuroscience.

Simultaneous whole-animal 3D-imaging of neuronal activity

cognitive functions and behavior, tools for non-invasive interrogation of neuronal circuits with high spatio-temporal resolution are required 1,2,3. Recent efforts have led to a number of approaches for 3D neural activity imaging, taking advantage of exciting chemical and genetically encoded fluorescent reporters 4,5,6.

APharmacologicalApproachtoDissectingthe

size of its nervous system allowed for the construction of a precise map of its neural origins and connections. This, in conjunction with a vast repertoire of mutant libraries and tools for optical imaging and interrogation of circuit electrophysiology, makes C. elegans an excellent model in

Multimodal fast optical interrogation of neural circuitry.

targeted together to Caenorhabditis elegans muscle and cholinergic motor neurons to control locomotion bidirectionally. NpHR and ChR2 form a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits.

Functional Analysis of the Motor Circuit of Juvenile

elegans motor circuit show that structurally divergent neural circuits can functionally converge on the functional output, and that neurons can multiplex. This study further demonstrates a larger role of functional communications devoid of ultrastructural features in a developing neural circuit.

Program Director/Principal Investigator (Last, First, Middle)

Guo ZV, Hart AC, Ramanathan S Optical interrogation of neural circuits in Caenorhabditis elegans Nature Methods 2009 6: 891-96. PMID: 19898486 Jeong H, Then F, Savas J, Tanese N., Hart AC, Krainc D Acetylation Targets Mutant Huntingtin to Autophagosomes for Degredation Huntington s Disease. Cell 2009 137(1):60-72. PMID: 19345187

Optical interrogation of neural circuits in Caenorhabditis

optical interrogation of neural circuits in Caenorhabditis elegans. Zengcai V Guo. 1 3, Anne C Hart. 4 & Sharad Ramanathan. 1 3. used to monitor neural activity. 9,10, and there have been notable advances in the use of light-gated cation and chloride channels (channelrhodopsin-2 (ChR2) 11, Volvox carteri channelrhodopsin (VChR1) 12. and

Stimulating Cardiac Muscle by Light - AHA/ASA Journals

ChR2 current in HEK cells,20 Caenorhabditis elegans muscle cells,10 or in cardiomyocytes.13 For example, for comparable irradiance levels, at holding potential of 40 mV, about 20-times-higher steady-state current densities were measured in our donor cells compared with ventricular myocytes from a trans-

Màster en optogenètica, quimiogenètica i biofotònica

Genetic and optical targeting of neural circuits and behavior zebrafish in the spotlight Curr Opin Neurobiol, 19, p. 553-560. Keynote lecture per Gero Miesenbock (Oxford). El professor Miesenbock, professor de fisiologia i director del Centre for Neural Circuits and Behaviour de la

研究员 - SJTU

姓名 郭增才 职称 研究员 荣誉称号 中组部青年千人 联系方式 [email protected] 研究工作 简历 2002 清华大学 工程力学学士

NEUROSCIENCE Near-infrared deep brain stimulationvia

NEUROSCIENCE Near-infrared deep brain stimulationvia upconversion nanoparticle mediated optogenetics Shuo Chen, 1* Adam Z. Weitemier, Xiao Zeng,2 Linmeng He, 1Xiyu Wang, Yanqiu Tao, 1Arthur J. Y. Huang, Yuki Hashimotodani,3 Masanobu Kano,3,4

ARTICLES - Stanford University

Caenorhabditis elegans muscle and cholinergic motor neurons to control locomotion bidirectionally.NpHR and ChR2 form a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits. To enable precise perturbation of living circuits, we recently

Selected publications

Gottschalk, and K. Deisseroth (2007) Multimodal fast optical interrogation of neural circuits. Nature 446:633-639 Schroll C, Riemensperger T, Bucher D, Ehmer J, Voller T, Erbguth K, Gerber B, Hendel T, Nagel G, Buchner E, Fiala A. (2006) Light-induced activation of distinct modulatory neurons triggers appetitive or aversive learning

A 3D glass optrode array for optical neural stimulation

A 3D glass optrode array for optical neural stimulation T.V.F. Abaya,1 S. Blair,1,2,* P. Tathireddy,1 L. Rieth,1 and F. Solzbacher1,2 1Department of Electrical and

Circuit interrogation in freely moving animals

encoded optical indicators have allowed direct visualization of neural activity in behaving Caenorhabditis elegans1,2 and larval zebrafish3 5. Recent technological advances are extending these recordings to more neurons, more behaviors, and even more animal models. Brain and behavior can be studied right out of the box in a growing

Three-wavelength light control of freely moving Drosophila

optical method is a lack of specificity between various neurons; however, state-of-the-art genetically modified animals can help the optical method target specific neural circuits. Therefore, the resolution and specificity can be simultaneously satisfied in optical-based systems.

Optical interrogation of neural circuits in Caenorhabditis

Optical interrogation of neural circuits in Caenorhabditis elegans Zengcai V Guo, Anne C Hart & Sharad Ramanathan Supplementary figures and text: Supplementary Figure 1 Direct and indirect synaptic connections from ASH to AVA and AVD. Supplementary Figure 2 Neuronal activity in AVA correlates with backward movement.

simultaneous all-optical manipulation and recording of neural

flexible and long-term optical interrogation of functionally defined neural circuits with single-cell and single-spike resolution in the mouse brain in vivo. Optogenetic actuators are rapidly revolutionizing experimental manipulations of neural activity, enabling activation and inactiva-

Optical Technologies Expand Vistas Into the Brain

optical remote to zero in on specific types of neurons. Electrodes, in contrast, are much less selective; they stimulate all the cells in a region. With these advantages, the optical remote has wide potential application for addiction research as well as basic neuro-science. This tool can help scientists see how neural circuits go awry in

Simultaneous whole-animal 3D imaging of neuronal activity

tools for non-invasive interrogation of neuronal circuits with high spatio-temporal resolution are required 1, 2. A number of approaches for 3D neural activity imaging, taking advantage of chemical and genetically encoded fluorescent reporters 3,4 exist. While some or are based

Novel Technological Advances in Functional Connectomics in C

in computational modeling, which allows for interrogation of both local and global neural circuits, leading to different behaviors. Combining these approaches will shed light into how neural networks process sensory information to generate the appropriate behavioral output, providing a complete understanding of the worm nervous system.

Microfluidics for Electrophysiology, Imaging, and Behavioral

48 increased throughput for chemical, optical, and electrical interrogation of C. elegans and D. melanogaster 49 on microfluidic platforms. This confluence of technologies has revealed how many behaviors can be 50 implemented by neural circuits,20 24 however, C. elegans and D. melanogaster may not be the best suited