Chloride Dependence Of Growth In Bacteria
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Saline Systems BioMed Central
The moderate halophile Halobacillus halophilus is the paradigm for chloride dependent growth in prokaryotes. Recent experiments sh ed light on the molecu lar basis of the chloride dependence that is reviewed here. In the presence of moderate salinities Halobacillus halophilus mainly accumulates glutamine and glutamate to adjust turgor.
DISCOVERY OF ENZYMES RESPONSIBLE FOR AN ALTERNATE MEVALONATE
prevents the growth of H. volcanii cells in vivo suggesting the essentiality of the enzyme and the mevalonate pathway in these microbes. H. volcanii also encodes (HVO 2762) an isopentenyl monophosphate kinase (EC
Fighting Scale Removal and Prevention
tal growth, and also implies that small seed crys-tals may redissolve.2 Thus, given a large enough degree of supersaturation, the formation of any seed crystal will encourage an increase in the growth of scale deposits. The seed crystal, in effect, is a catalyst for scale formation. Crystal growth also tends to initiate on a pre-
Sugar- and salt-tolerant yeasts
sodium chloride, although they grew well in a pH range of 34-66 in 1 moll-' sodium chloride (Onishi 1957b). Such different pH dependence of growth was thought to be one of the phenomena that reflected the differences between sugar-tolerance and salt-tolerance of yeasts. Restaino et al. (1983) reported that preconditioning of Z.
Response of single bacterial cells to stress gives rise to
rise to complex patterns of history dependence at the population level. Cells were exposed to two events of sodium chloride exposure, the warning event and stress event. We varied the time between the two events ( A), the concentration of the warning event ( B), and the duration of the warning event ( C) in a series of experi-ments.
Studies on the filamentous gliding bacteria Vitreoscilla
2.4.5 The effect of sodium chloride on growth 64 2.4.6 The effect of FCCP and monensin on growth 65 2.4.7 The effect of potassium on growth 65 References 67 Chapter 3 Purification of cytochrome bo from Vitreoscilla strains LB13 and C1 3.1 Introduction 69 3.2 Materials and methods 72 3.2.1 Overview of the purification process 72
Perchlorate reduction by microbes inhabiting oil reservoirs
not split into chloride and molecular oxygen as occurs in bacteria. Rather, chlorite reacts chemically with sulﬁde, forming oxidized sulfur compounds, which are reduced to sulﬁde in the electron transport chain by the archaeon. The dependence of perchlorate reduction on sulfur compounds could be shown.
FACULTY OF NATURAL SCIENCES CONSTANTINE THE PHILOSOPHER
Dependence of the number of bacteria over time is expressed in chart shown in Figure 1. Figure 1: Graph expressing dependence the number of bacteria and the time of growth Solve the following tasks using the graph shown in Figure 1: a) Find the number of bacteria N t present in culture after 12 hours.
Isolation And Characterization Of Heavy Metal Resistant
favourable growth condition for the bacteria, which include adjustment of pH, neutralization of residual chlorine and reduction of DO in supersaturated samples. Sample is then diluted and the appropriate amount of seed bacteria added. Initial dissolved oxygen content is recorded and the
resource dependence of bacterial C : N : P stoichiometry
bacteria, we measured biomass C, N, P and nucleic acid content simultaneously in E. coli K-12 under a wide range of substrate C : P and growth rates. We also surveyed the literature on the homeostasis of biomass C : P and N: P for cultured and natural freshwater lake bacteria, and compared these with the results of our experiments.
Corrosion of copper and steel alloys in a simulated
extract (0.5 g L 1), peptone (0.3 g L 1), and sodium chloride (1 g L 1) in distilled water  at pH 6. Ethanol (5% by volume) was added as a carbon source. A concentrated Acetobacter culture was established by growing the bacteria in 100 mL of culture media for 1 week. This established culture, which is in a stationary
Influence of Media Composition on the Growth Rate of
Nitrogen source dependence was studied comparing KNO3 (M-8 media) and urea. Various urea loadings were testing to determine optimal levels of urea addition. Different EDTA sources were tested and compared to the growth rate without EDTA. Vitamin B and micronutrients (B, V, and Mo) were added to determine if these additions could enhance growth.
degrading marine bacteria: Acyl-homoserinelactone 0 they
EFFECT OF VAI CONCENTRATION ON GROWTH. Since it was ambiguous whether VAI could serve as a sole carbon source in certain bacteria, a VAI concentration dependence experiment was performed. A pH 7, buffered SW base with ammonium chloride was used, and various amounts of VAI were added. The
A Look at Transformation Efficiencies in E. coli An
described bacteria (Esherichia coli) as preferentially interacting with, and taking up double-stranded DNA (4). Hanahan also described the existence of a transitory state of competence for transformation, which is generally related both to conditions of growth and to the circumstances under which the cells and DNA are combined. It is
Quantitative and Physiological Analyses of Chloride
not determined whether growth failure was due to a lack of chloride or to a growth inhibition by sulfate, quantitative mea-surements were not done, and the physiology of the Cl2 de-pendence was not analyzed. To unequivocally identify a chlo-ride dependence of the salt marsh bacterium H. halophilus,we performed a detailed physiological
Mechanisms of plant response to salt and drought stress and
decrease (in tolerant species) chloride uptake. Root pruning and defoliation showed a dependence of the uptake on root system size. Leaf biomass was responsi-ble of leaf chloride accumulation. At a cellular level the osmotic stress, caused by salt and drought, leads to different degrees of effects de-pending on the severity of the stress
Are bacteria more like plants or animals? Growth rate and
bacteria, we measured biomass C, N, P and nucleic acid content simultaneously in E. coli K-12 under a wide range of substrate C : P and growth rates. We also surveyed the literature on the homeostasis of biomass C : P and N : P for cultured and natural freshwater lake bacteria, and compared these with the results of our experiments.
EG0800299 Antibacterial Effect of Acrylic Acid-Grafted Cotton
affected the growth of bacteria on wool samples more than on polyester fabric. The dependence of the growth of S. aureus bacteria (N) on AA graft yield (GY) is shown in Fig. 3 for the different fabrics. The N-GY relationship is made at a constant nutrient time of 24h and 303 K, temperature.
Active versus inactive bacteria: size-dependence in a coastal
dye CTC (5-cyano-2,3-ditolyl tetrazolium chloride). We also compute the contribution of each size class to total bacterial production and provide evidence of the size dependence of bacterial specific growth rates. MATERIALS AND METHODS Sampling area and experimental setup. We sampled a natural plankton community in the Bay of Blanes,
Molecular Mechanisms of Adaptation of the Moderately
3. The Chloride Modulon One of the outstanding, or even to our knowledge, unique physiological features of H. halophilus is its chloride dependence of growth, gene expression and enzymatic activity. Growth of H. halophilus was shown to strictly depend on the presence of chloride, in absence of chloride the strain does not grow.
Temperature and Corrosion Rate More Complex Than You Think
With chloride containing environments and stainless steel, there is usually a threshold temperature at which pitting occurs (penetration of the passive film leading to severe localized attack). Since a pit is a location where solution is trapped and is ever changing compared with the bulk process environment, the rate of propagation may
Autoinducer-2-Producing Protein LuxS, a Novel Salt- and
chloride dependent. Maximal accumulation of LuxS was observed at 0.5 to 1.0 M Cl and depended on the salinity. The moderately halophilic bacteria are a specialized group of organisms that are strictly salt dependent for growth. About 0.5 M of NaCl is required for optimal growth, and most inter-estingly, cells are able to grow over a rather wide
Growth of Lactic Acid Bacteria on Gold Influence of Surface
Dec 13, 2020 particular bacteria strain and within bacteria phenotype [21,22]. It was found that the structure of a cell wall of Lactobacillus genera may vary, which strongly a ects cell host interactions . What is more, Polak-Berecka et al.  investigated the dependence between the cell envelope components of
Motility and Flagellum Synthesis in Halobacillus halophilus
Most interestingly, growth of vegetative cells as well as germi-nation of endospores of H. halophilus is strictly chloride de-pendent (1a, 4). H. halophilus is the ﬁrst obligate chloride-dependent bacterium known to date, and projects to study the physiological basis of the chloride dependence have just begun.
Formation of natural biofilms during chlorine dioxide and u.v
(hardened polyethylene, polyvinyl chloride, steel and copper) were used as substrates for bioﬁlm formation. Apart from young bioﬁlms, several months old bioﬁlms were compared in terms of material dependence, biomass and physiological state. Vital staining of bioﬁlms with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and the DNA-
Technical Note: Ennoblement A Common Phenomenon?
the time dependence of E corr for brass exposed to AS (Figure 1) and LB medium (Figure 2) as well as for Al 2024 exposed to AS (Figure 3) and LB medium (Figure 4). AS was prepared from Väätänen nine salts solution (VNSS) (17.6 g/L sodium chloride [NaCl], 0.08 g/L sodium bicarbonate [NaHCO 3], 0.04 g/L potassium bromide [KBr], 0.41 g/L
Effect ofChanges Osmolarity Growth Medium
in the osmolarity ofthe growth mediumalso regulated the expression ofthe outer membraneproteins. This regulation wasabolishedif V. cholerae cells weregrownin Pi-depleted medium.Analysisofthelytic behavior and composition of outer membrane proteins of an osmotically fragile mutant strain revealed a similar dependence onthe osmolarity ofthe
Reduced dependence of rhizosphere microbiome on plant-derived
Reduced dependence of rhizosphere microbiome on plant-derived carbon in 32-year long-term inorganic and organic fertilized soils Chao Ai a, Guoqing Liang a, Jingwen Sun a, Xiubin Wang a, Ping He a, b, Wei Zhou a, *, Xinhua He a, c, d, *
Screening and Characterization of Exopolysaccharide Substance
Jun 11, 2020 colony producing bacteria. 2.2 Isolation of Exopolysaccharide producing microorganisms: Isolation of exopolysaccharide producing microorganisms were done using nutrient agar plate (Peptone 10.0gm, Beef extract 3.0gm, Sodium chloride 5.0gm, Agar powder 30gm, Dissolved in 1000 mL of Distilled water, pH 7.0) .
Fertilizer Industry Handbook 2018 - Yara
2016) consumption growth rates were 1.4% for nitrogen, 1.2% for phosphate and 2.3% for potassium. Going forward, The International Fertilizer Association (IFA) forecasts nitrogen fertilizer demand growth at 1.1% per year through 2021. A growth rate of 1.6% a year is estimated for phosphate and 2.2% for potassium.
The Hematological Complications of Alcoholism
especially those that defend the body against invading bacteria. Consequently, alcoholics frequently suffer from bacterial infections. Finally, alcohol adversely affects the platelets and other components of the blood-clotting system. Heavy alcohol consumption thus may increase the drinker s risk of suffering a stroke. KEY WORDS:
Optimization of Fermentation Factors for Polyhydroxybutyrate
both glucose and xylose from the simulated hydrolysate. PHB was also confirmed as a growth-associated product of the bacteria, with observable patterns of catabolite repression and diauxic growth. Optimum conditions that maximize biomass concentration (7.3 g/L), PHB concentration (3.91 g/L), and substrate
Rapid Identiﬁcation of Bacteria from Positive Blood Cultures
cation of pathogens can result, primarily due to the dependence on growth-based identiﬁcation systems. To address these limitations, we took advantage of terminal restriction fragment (TRF) length polymorphisms (T-RFLP) due to 16S ribosomal DNA (rDNA) sequence diversity to rapidly identify bacterial pathogens directly from positive blood culture.
HIGHLY IMMERSIVE DIGITAL SCIENCE LABS
Precipitation Titration of Cobalt Chloride Temperature Dependence of Salt Solubility Stoichiometry of Chemical Reactions Decomposition of Malachite Empirical Formula of a Hydrate Empirical Formula of Copper Oxide Limiting Reactant and Excess Reactant Mole to Mole Relationship Between Cu and Ag
Effect of Sodium Chloride on Ferrous Iron Oxidation and
NaCl 300 mM strongly affected cell viability, growth and iron oxidation of Sb. thermosulfidooxidans Outlook Effect of NaCl on sporulation of Sb. thermosulfidooxidans Effect of NaCl on Sb. thermosulfidooxidans in dependence of other substrates (sulfur, pyrite) Biofilm formation and Bioleaching of metal sulfides by Sb
Initial adhesion and surface growth of Pseudomonas aeruginosa
growth of the adhering bacteria using nutrient broth as growth medium. Initial adhesion was twice as fast on the positively charged methacrylate than on the negatively charged copolymer. Surface growth, however, was absent on the positively charged copolymer, while on the negatively charged methacrylate the number of bacteria increased
Using ABA to Address Feeding Cigna Autism Awareness
Failure to thrive or stunted growth Tube dependence Thiamine Chloride Hydrochloride, Pyridoxine Hydrochloride, good bacteria Bone broth