Type II Collagen Matrices In Cartilage Repair

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Cartilage-specific type II collagen were observed by IHC and SDS-PAGE in all constructs, No collagen types I or type X were detected. Figure 1: Alcian -blue staining of A: P(L)LA, B: P(L/D)LA, C: Col HA seeded constructs. Figure 2: Ultrastructure photographs 2A: Healthy chondrocyte in the

Articular cartilage repair with recombinant human type II

for cartilage repair as periosteal flap substitutes in ACI.5 8 Our research group has developed a novel biomaterial scaffold rhCo-PLA, which is a biodegrad-able scaffold combining recombinant human type II collagen (rhCo) and polylactide 96/4 felt (PLA). Being free of animal products, rhCo-PLA scaffold eliminates


Articular cartilage tissue engineering is a useful tool to study and enhance the wound healing processes of articular cartilage in vivo. Current tissue engineering scaffolds for articular cartilage are produced by cross-linking type II collagen with glycosamino-

Effects of Biochemical and Mechanical Stimulation of

loading experiments have been done, however, with type II collagen-GAG scaffolds cultured in serum-free medium. Chapter 3 describes a series of experiments in which chondrocyte-seeded scaffolds were subjected to dynamic compression and the effects of this treatment on the proliferation of the chondrocytes, their synthesis of ECM, and the

Genetically Engineered, Enzymatically Crosslinked Elastin

the presence of collagen types I and II as early as 2 weeks of culture. As shown in the figure, virtually all cells in a section stained for type II collagen, whereas a much smaller fraction of the cells were positive for type I collagen. This observation was consistent over the 6 wee k sampling period.


Collagen II <12 hr CAC/ Collagen II 4 wk % Original Defect Area Cultured Autologous Chondrocytes Hyaline Fibrocartilage Fibrous HA Breinan, et al. J Orthop. Res. 2000;18:781-789 and C.R. Lee, et al. J. Orthop. Res. 2003;21:272-281 No diff. after 12 mo. Implantation of Cells Alone or in a Type II Collagen Matrix Conclusion: A cell-seeded matrix is

Insulin-like growth factor-I enhances cell-based repair of

expression of large proteoglycans and type-II collagen, without stimulating the synthesis of type-I collagen or inducing dedifferentiation of chondrocytes to a fibroblast-like cell shape.39,40 In addition to the anabolic effects of IGF-I on normal articular cartilage, there is evidence that IGF-I is beneficial to injured cartilage. The

Orthopaedic Research Society

large areas of type II collagen-containing matrix between them, similar to the organization of hyaline cartilage (Figure 1). This was less evident in chondrocyte pellets which also displayed less distinct type VI pericellular labelling. After 7 weeks, the hyaline cartilage-like appearance of the chondron

Comparison of Engineered Peptide-Glycosaminoglycan

Jul 17, 2015 the peptide component. For Construct II, to the template FVC assemblies we incorporated a short peptide sequence KLGFFAH (abbreviated as: CP) followed by Type II collagen (Col-II), Ch and CS. It was recently reported that chondrocytes express specific collagen Type II-binding integrin protein. The CP

RESEARCH ARTICLE Open Access Evaluation of chitosan-GP

Keywords: Chitosan-GP, Cartilage, Proteoglycan, Type II collagen, Equine joint, Biocompatibility, Scaffold Background Injuries to articular cartilage are common, and may cause pain, disability, impairment, and early retirement of athletes. Once damaged, articular cartilage has very little capacity for spontaneous healing, and many repair tech-

Regeneration of Hyaline Cartilage Using a Mechanically-Tuned

For the formulations in Table 1, porous collagen matrices can be prepared by incubation of 0.8 g (or 0.4 g) of insoluble type II collagen in 50 ml 0.5 M acetic acid (pH 2.5) at 4˚C for 16 hr, adding 50 ml of ice-cold DI water, and homogenizing the mixture in a blender. The collagen is then filtered (using 90 m nylon gauze filters), μ


Type II collagen has been identified as an important component in regeneration of articular cartilage, with a significant role in the growth and the proliferation process of chondrocytes. Thus, type II collagen or materials based on type II collagen are usually preferred in the treatment and research of the cartilage regeneration [19].

W O N D E R W H Y ? The Deterioration of Articular Cartilage

fraying and fibrillation of the articular cartilage surface. (See Figure 3.) This coincides with a loss of proteoglycans from the matrix of articular cartilage.12 Articular cartilage contains chondrocytes embedded in an extracellular matrix composed primarily of type II collagen and proteoglycans.

Nanomechanics of the Cartilage Extracellular Matrix

the cartilage extracellular matrix is organized into pericellular, territorial, and interterritorial matrices, each of which is present at increasing distance from the chondrocyte cell surface. Panel b adapted with permission from Reference 34.

Cartilage Tissue Engineering-A Novel Biomaterial for

Figure 1: A self-assembled cartilage like complex from hyal-uronic acid (HA), aggrecan (AG), and type II collagen. We demonstrated that it is possible to create a novel biomaterial for cartilage repair with high performance as well as microstructures comparable to hyaline articular cartilage (Figure 1).

Col2â GFP reporter marks chondrocyte lineage and

procollagen II. Figure 6 shows that intensity of fluores-cence correlated well with the level of procollagen II synthesis. Thus, fluorescence derived from the Col2-GFP trans-gene provides an in situ cellular assay for type II col-lagen synthesis. One can assess the level of type II collagen synthesis of one or a group of cells relative to

US006352558B1 (12) United States Patent (io) Patent No.: US

Meniscus Cells Seeded in Type I and Type II Collagen-Gag Matrices , 44th Annual Meeting, Orthopaedic Research Society, Mar. 16-19, 1998, New Orleans, Louisiana. S. Nehrer et al., Chrondrocyte-Seeded Type I and Type II Collagen Implants Investigated in Vitro , Fifth World Bio­ materials Congress, May 29-Jun. 2, 1996, Toronto, CA.


SEEDED COLLAGEN MATRIX 4 mm Chondrocyte-seeded type II collagen type II collagen implant* * Cells seeded into the matrix * Cells seeded into the matrix 24 hours* and and 4 weeks prior to implantation * HA Breinan, * HA Breinan, et al. J Orthop. Res. 2000;18:781-789 and C.R. Lee, and C.R. Lee, et al. J. Orthop. Res. 2003;21:272-281 281


or porcine collagen, thus they all produce unwanted fibrous cartilage and bear the risk of passing diseases like BSE. The new matrix from jellyfish collagen shows important advantages over other matrices. This collagen has similarities to vertebrate collagen-type II, the main type in healthy hyaline cartilage.

REVIEW Mesenchymal stem cells in the treatment of traumatic

tilage repair tissue formation to be assessed within simu-lated defects in controlled in vitro environments. Porcine MSCs embedded in agarose gel implanted within chondral explants showed an abundance of type II collagen and gly-cosaminoglycan (GAG) matrix after 6 weeks of culture [35]. Similarly, human MSCs embedded in alginate gel and


having collagen type X, which helps cartilage mineralization and provides structure integrity.36 From a matrix point of view, articular cartilage is classified into three regions including territorial, interterritorial, and pericellular matrices based on their distances from the cells.


use of decellularized cartilage bovine can serve as scaffold to support proliferation dan differentiation of the stem cell. Purpose: This research is to compare the effect of decellularization technique on collagen type II and matrices porosity of cartilage bovine scaffold.

Dynamic Compressive Loading Improves Cartilage Repair in an

this fibrocartilaginous repair tissue and its poor integra-tion with the surrounding hyaline cartilage. To enhance the quality of the newly formed tissue, vari-ous augmentation techniques using synthetic collagen matrices,scaffolds, ordevices havebeen developed.8,13 How-ever, procedures aimed at augmentation of microfracture,

Bioactive IGF-1 release from collagen GAG scaffold to enhance

sively investigated for use in articular cartilage repair [10, 12, 17, 34, 40, 51]. Fortier et al. [12] demonstrated that the addition of 10 100 ng/ml IGF-1 enhanced the levels of proteoglycan and type II collagen synthesised by chon-drocytes seeded in fibrin matrices and that the cells main-tained their phenotype in vitro. In addition, IGF

The reactions of articular cartilage to experimental wounding

native, type II collagen (23) and has been used to show, immunohistochemically, the presence of denatured type II collagen in human osteoarthritic cartilage (24). Antibody BC-13 recognizes the new C-terminus of the aggrecan molecule, which is left bound to the cartilage extracellular matrix after cleavage by aggrecanase enzyme activity (25).

Autologous Matrix-Induced Chondrogenesis © The Author(s) 2014

mercially available collagen and alginate scaffolds that have been used for cartilage repairs that are reported in the literature.32,33 ChondroGide (Geistlich Biomaterials, Switzerland) The porcine-derived type I/III collagen membrane ChondroGide is the commonest type of matrix used. This is a protein-based natural bilayer collagen matrix that

RESEARCH ARTICLE Open Access An ovine in vitro model for

antibody (rabbit anti-human type II collagen, Acris). Sub-sequently, sections were processed using the EnVision System Peroxidase Kit (DAKO) according to the manufac-turer s recommendations, followed by counterstaining with hematoxylin (Merck). To verify the presence of type II collagen during expansion culture in monolayer, chon-

Development and characterization of a eukaryotic expression

type II procollagen can lead to diseases including achon-drogenesis, hypochondrogenesis and various skeletal dys-plasias [35]. Type II collagen matrices have been used to support cell growth and have proven particularly useful for promoting proliferation of chondrocytes, which are important for repair of damaged cartilage [28, 29, 36 38].

Characterization of a biomaterial with cartilage-like

Jul 01, 2018 Key words: Cartilage, Osteoarthritis, Bioengineering, Cartilage Repair, Type II collagen, Type X collagen, Calcification, Chondrocyte. Introduction Cartilage is a highly specialized connective tissue that consists of relatively few cells embedded in an abundant extracellular matrix. The extracellular matrix of cartilage

ournal of Pugliano et al, J Stem Cell Res her 21, :4 Stem

to develop a new therapeutic implant for cartilage repair by combining jellyfish collagen type II as an implant, to avoid the collagen type I or III of mammalian origin, growth factors TGF-β3 and adult mesenchymal stem cells derived from bone marrow. To functionalize our jellyfish implant with TGF-β3, we use our

Articular cartilage tissue engineering: the role of signaling

disrupts cartilage differentiation both in vitro and in vivo by inhibiting condensation [22 24]. In addition to the role in condensation, TGF-b stimulates cell proliferation and synthesis of cartilage matrix such as glycosaminoglycans (GAG) as well as expression of cartilage-specific genes such as aggrecan and type II collagen [25, 26]. TGF-b-

A Novel Biomaterial for Cartilage Repair Generated by Self

Type II collagen (Collagen Research Center, Inc., Tokyo, Japan) was dissolved in DDW at final concentrations of 0.1, 0.25, 0.33 or 0.5 mg/ml at room temperature. The pHs of the AG + HA solutions and the collagen solutions were adjusted to values from 4.0 to 11.0 in increments of 0.5. Then, equal volumes of the AG + HA and the collagen solutions

Monomeric, porous type II collagen scaffolds promote

type II collagen, compared to type-I and pure type-II scaffolds. We conclude that collagen type-II and CS can be used to promote a more chondrogenic phenotype in the absence of growth factors, potentially providing an eventual therapy to prevent OA. Osteoarthritis (OA) is a major cause of disability in the adult population1. Traumatic lesions

A Col I and BCP ceramic bi-layer scaffold implant promotes

Collagenous matrices provide a lot of biological functions to maintain cell and growth factor transport into the cartilage lesion.24 Thus, it is important to develop an adequate collagen framework in cartilage repair. Although the cartilage matrix mainly contains type II collagen (Col II), Rutgers et al. reported that no signi cant differences

Chondrogenic commitment of human umbilical cord blood-derived

transcription factor SOX9 promotes the transcription of genes encoding cartilage matrix proteins such as type II collagen and aggrecan 10. Alternative splicing of type II collagen mRNA leads to two type II procollagen isoforms. The IIA isoform is expressed in early chondrogenesis, and the IIB isoform is expressed in mature articular carti-lage11.

Marrow stimulation and chondrocyte transplantation using a

The porous layer consists of type II collagen and serves the cell-seeding process. Unseeded and chondrocyte-seeded matrices have been tested and pre-sented in previous in vitro studies conducted by our group; porosity was shown to be 67% in the type I/III layer and 83% in the type II collagen layer22,23. The actual manufacturing

Hybrid and Composite Scaffolds Based on Extracellular

these three cartilage types. Hyaline cartilage is the most abundant and well characterized, found as AC on the sur-faces of bones.2,3 AC can bear loads of up to around 20MPa during normal joint movements.1 The major macromolecular components of hyaline cartilage include collagen (COL) type II and X, cartilage oligomeric matrix protein, and

Collagen: Animal Sources and Biomedical Application

tendon reinforcement, skin and wound healing and hernia repair. Type I and II collagen is isolated from equine skin, articular cartilage and flexor tendon (Cortial et al., 2006). Collagen type I,II,III and V were obtained from chicken neck among which type I was predominant.