The Action Of Erythropoietin On Erythroid Cells In Vitro

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Erythropoietin: Current Status

FIG. 1. Differentiation and maturation of erythroid cells. Erythroid progenitor cells are derived from pluripotent stem cells shown on left. Under the influence of Epo, committed erytbroid progenitor cells progress through the BFU, and CFU, stages into proerythroblasts, the earliest recognizable red cell precursor inthebonemarrow.

Some Studies of Erythroid Differentiation in vitro

The pattern of erythropoietin action during erythrocyte differentiation:ER cell erythroid cells. In the scheme shown in Figure 1, epo may act on the original stem Most of our studies of marrow cells in vitro have involved short periods, and have

A functional erythropoietin receptor is necessary for the

murine erythroleukemia cells; that TPO has a mitogenic effect on normal murine splenic erythroid precursors; and that neutralizing antibodies directed against the EPO-R [25] abrogate roughly half of TPO s effect on erythroid cells. Taken together, these and other data from our laboratory led us to hypothesize that TPO may be acting on erythroid cells,

Interaction of inflammatory cytokines and erythropoeitin

ropoiesis is effected by erythropoietin (Epo). Epo is pro- duced mainly by the kidneys (16) but also extrarenally by macrophages (17,18). Proliferation and haemoglo- binization of erythroid progenitors is inducible by Epo in vitro (19). Inappropriately low serum Epo levels have

ASN Neuro Erythropoietin as a Neuroprotective Volume 11: 1

Erythropoietin (EPO) is a cytokine mainly induced in hypoxia conditions. Its major production site is the kidney. EPO primarily acts on the erythroid progenitor cells in the bone marrow. More and more studies are highlighting its secondary functions, with a crucial focus on its role in the central nervous system.

Granulocyte-Macrophage Colony - Stem Cells Journals

(CFU-MK) proliferation. Its action, however, does not extend to more mature erythroid and megakaryocyte cells suggesting the need for com- bined treatment with lineage-specific growth fac- tors such as erythropoietin (Epo) or IL-6 to obtain a complete myeloid stimulation. When GM-CSF is discontinued, its action rapidly vanishes, and a

Non-erythroid activities of erythropoietin: Functional

Erythropoietin (EPO) is the key regulator of erythropoiesis. Secretedmainlybythekidney,EPOpromotesthesurvival,prolifer-ation, and differentiation of committed erythroid progenitor cells (Sasaki et al., 2000). Recombinant human EPO (rHuEPO) has thus been adopted for the treatment of various types of anemia (Cheer

Ineffective erythropoiesis with reduced red blood cell

Little is known regarding the mechanism of action of 5-HT during hematopoiesis, and only scattered in vitro reports have described the influence of 5-HT on RBCs or the erythropoietic function (22, 23). Recently, an in vitro study showed that 5-HT enhances the

Mechanism of erythropoietin action on the erythroid

mining the mechanism of action of erythropoietin on these erythroid progenitor cells have been described, no conclusive evidence has yet been obtained because in most of the work a mixed population of bone marrow cells was used, thus limiting the analysis. We have demonstrated that a murine erythroid cell line TSA8 (Shibuya & Mak, 1983) can be induced to

Aconitase Regulation of Erythropoiesis Correlates with a

Background:Erythroid development requires the action of erythropoietin (EPO) on committed progenitors to match red cell output to demand. In this process, iron acts as a critical cofactor, with iron deficiency blunting EPO-responsiveness of erythroid progenitors.

Concise Review: Stem Cell-Derived - Stem Cells Journals

cytes. This process is positively regulated by erythropoietin (EPO). EPO exerts its action by binding to a specific receptor (EPO-R) expressed on both erythroid progenitor and precursor cells. At the progenitor cell levels, EPO-EPO-R binding acti-vates proliferation while at

Molecular Mechanisms in Erythroid Differentiation

mediated through erythropoietin which is produced in the juxtaglomerular cells of the kidney in response to anoxia and promotes the maturation of erythroblasts. It has been possible to purify erythropoietin at least partially and to demonstrate its effects on cultured erythroid tissue in vitro

Regulation of Erythropoietin Receptor Activity in

superfamily [3]. The EPO receptor (EPOR) is expressed at high levels on erythroid progenitor cells and is the primary target for EPO binding [4]. However, EPORs have also been detected and demonstrated to be functionally active on non-hematopoietic cells, including endothelial cells

Erythropoietin directly remodels the clonal composition of

20/4/2020  The cytokine erythropoietin (EPO) is a potent inducer of erythrocyte development and one of the most prescribed biopharmaceuticals. The action of EPO on erythroid progenitor cells is well established, but its direct action on hematopoietic stem and progenitor cells (HSPCs) is still debated. Here, using cellular barcoding, we

Mechanism of Action of Androgens on Erythropoiesis A Review

regardless of their effect on erythropoietin secretion. In mice, androgens increase not only hemoglobin levels but also the number of granulocytes3. In vitro androgens enhance hematopoiesis in bone marrow cultures and stimulate the erythroid as well as the myeloid lines in semi-solid cell cultures4,5. Subsequently clinical evidence

Critical Review - Wiley

4/12/2008  matopoietic cells lines, like G1E, were also established and used in delineating transcriptional regulation upon erythropoiesis. G1E cells are an immortalized GATA-1-null erythroblast line derived from gene-targeted embryonic stem cells (21). This sys-tem has been extensively used to uncover specific signaling pathways regulating erythropoiesis.

NACA is a positive regulator of human erythroid-cell

and the effect of NACA overexpression in such cells generated in vitro from human-cord-blood-derived CD34+ hematopoietic cells. We observed: (i) that NACA is maintained during in vitro erythroid differentiation of these cells but that, in marked contrast, its expression is suppressed during their megakaryocytic or granulocytic differentiation; (ii) that

Red cells I: inherited anaemias - The Lancet

Red cells are the products of haemopoietic stem cells. The development of in-vitro clonal assays for progenitor cells GATA-2 Erythropoietin Erythroid Krüppel-like factor Nuclear factor-erythroid 2 Figure 2: biliverdin by the action of biliverdin reductase. An

An activin receptor IIA ligand trap promotes

cell factor (SCF, also termed KITLG) and erythropoietin (EPO) for the proliferation and survival of erythroid cells (von Lindern et al, 2004; Richmond et al, 2005). Anaemia, as defined by a decline in circulating red blood cells (RBCs), is one of the most common haematological pathologies in both adults and children. Treatment of anaemia ranges from die-

Nuclear Condensation during Mouse Erythropoiesis Requires

7/3/2016  openings were an artifact of in vitro culture. The number of cells with lamin B openings increased during in vitro culture and constituted approximately 20% 30% of the total fetal liver cells and bone marrow erythroid cells (Figure 1C). Mostof the cellscontained one opening (<5% of cellscontained two or more openings).

The role of carbohydrate in recombinant human erythropoietin

yielded erythropoietin with higher affinity to the receptor than the undigested hormone and therefore an increased in vitvo activity. Although erythropoiein from which N-linked or total sugars were removed also had higher affinity for the receptor, their in vitro activity remained unchanged compared with that of the undigested erythropoietin

Cell, Vol. 14, 733-740, July 1978, Copyright 0 1978 by MIT

commitment of erythroid stem cells (CFU-E) to form colonies which made HbC in vitro, and to detect the initial accumulation of pc-globin mRNA and the onset of HbC synthesis in erythroblasts in vivo. CFU-E-derived erythroid colonies were formed in plasma clot culture at a low erythropoi-

Rapid Communication Insights into the cellular mechanisms

cells, we have analyzed the effects of the combination of ery­ thropoietin (Epo) and thrombopoietin (Tpo) on the in vitro differentiation toward erythropoiesis and thrombopoiesis. The number of CD41+ cells that accumulated over 2 weeks of culture, as well as the number of globin+ cells in the same cul­

Effect of recombinant human erythropoietin on endothelial

endothelial cell growth and angiogenesis in vitro. The mechanisms are unknown. Because an increase in endothelial cell survival could play inhibit apoptosis in the erythroid progenitor cells [5 7]. a role in this process, we examined the effect of rHuEPO on lipopoly- In

An Erythroid Differentiation Signature Predicts Response

promoted erythroid differentiation of primary hematopoietic progenitor cells grown in vitro. Conclusions These studies indicate that lenalidomide-responsive patients have a defect in erythroid differentiation, and suggest a strategy for a clinical test to predict patients most likely to respond to the drug.

Erythropoietins: A common mechanism of action

avoidance of blood transfusion risks. Erythropoietin (EPO) interacts directly with the EPO receptor on the red blood cell (RBC) surface, triggering activation of several signal transduc-tion pathways, resulting in the proliferation and terminal differentiation of erythroid precur-sor cells and providing protection from RBC precursor apoptosis.

Simply red: A novel spectrophotometric erythroid

renders erythroid proliferation independent of erythropoietin and causes excessive red cell production [20,23]. In vitro methods for the generation of erythroid cells from hematopoietic stem cells derived from various sources have been established m and shown to yield both high proliferation of erythroid cells and produce functional, mature

Functional erythropoietin receptor of the cells with

The action of Epo on erythroid precursor cells has been only a generally accepted function; Epo supports survival of the cells and stimulates their proliferation and differentiation (see Ref. 1 for review). Epo, however, acts in vitro on other cells besides erythroid cells; Epo promotes differentiation of

Erythropoietin Stimulates Rise in Intracellular Free

Erythropoietin and granulocyte-macrophage colony-stimulat- ing factor (GM-CSF)stimulate the differentiation and prolif- erationof erythroid cells.To determine thecellular mechanism


Dörmer, 1976). Erythropoietin in vivo is defined as a substance, which is able to induce a burst of hemog!obin synthesizing cells in a po!ycythemic mouse that has no recognizable, immature erythroid cells. The erythropoietin is mainly pro­ duced in the kidney in responsetoa hypoxic stimulus (Mirand andPrentice, 1957).

The molecular mechanism of erythropoietin action

cern the mechanism of the action of EPO: the structure and function of EPO and its receptor; the biochemical effects of EPO on its primary targets, the erythroid-progenitor cells: the cellular responses which permit the regulation of erythroid- progenitor-cell differentiation into erythrocytes. Structure of

Erythropoietin and anemia of cancer

in vitro, appearas a burst of cells. They are appropri-atelynamedburstformingunits-erythroid(BFU-E). The late progenitors have a limited number of descendants, and in vitro they are surrounded by only small colonies, and are called colony forming units-erythroid (CFU-E). Maturation occurs grad-ually with sequential activation of erythroid trans-

Sox6 Is Necessary for Efficient Erythropoiesis in Adult

scarce in the developing erythroid cells and RBCs of Sox6 mutants and their presence appears inconsequential. Sox6 effectively upregulates the expression of multiple late erythroid cell markers, and one of its direct targets is Bcl2l1, which encodes Bcl-xL, an important survival factor acting downstream and beyond Epo signaling in erythroid cells.

ACE inhibitors and erythropoietin responsiveness

the response to erythropoietin is conflicting. 3-12 Various mechanisms have been offered to ex-plain why ACE inhibitors might affect the re-sponse to erythropoietin. First, it has been shown that angiotensin II directly increases the prolifera-tion of erythroid progenitor cells in vitro.13 Thus, any agent decreasing angiotensin II levels could

Induction of the Receptor for Erythropoietin in Murine

for the proliferation and differentiation of late erythroid pro genitor cells in vivo and in vitro. As with peptide hormones in general, these effects appear to be mediated through the inter action of the ligand with specific receptors on the cell surface of target cells. Recently, human urinary EPO was purified to


liver erythroid cells (Congote, submitted). This is not surprising, because IGF I has been found to stimulate the formation of murine erythroid cell colonies in defined culture media in the absence of erythropoietin (18). Although IGF I seems to have an action indistinguishable from erythropoietin in the murine system (18), and serum

Erythropoietin directly affects single hematopoietic stem

20/4/2020  The cytokine erythropoietin (EPO) is a potent inducer of erythrocyte development and one of the most prescribed biopharmaceuticals. The action of EPO on erythroid progenitor cells is well established, but its action on hematopoietic stem cells (HSCs) is still debated. Here, using cellular barcoding, we traced the

Primitive and definitive erythropoiesis in mammals

Erythropoietin (EPO) is a critical regulator of late-stage definitive, maturing erythroid cells. in vitro. Erythroid precursors are defined morphologically as proerythroblasts cell polarity through microtubule action, the formation of a con-tractile actomyosin ring, vesicle formation,

α4β1 integrin and erythropoietin mediate temporally

erythroid cells can be isolated from day 14.5 fetal liver based on the expression of CD71 and TER119 as previously described (Z hang et al., 2003). (B) CD71 and TER119 can be used as markers of erythroid differentiation in vitro. Day 14.5 TER119− fetal liver cells were cultured on fi bronectin-coated plates in

Functional Links Between Glucocorticoids and Cytokines In DBA

In vitro, GCs enhance the formation of murine erythroid colonies [23] and increase proliferation of erythroid cells in the presence of limiting amounts of erythropoietin (Epo) [24]. Interestingly, GCs also stimulate erythropoiesis indirectly by increasing Epo production in the kidney [25].

Erythropoietic Kinetics in Sheep Studied Means ofInduced

various times in bone marrow erythroid cells in-cubated in vitro with 59Fe. The changeover from HbAto HbCformation lagged by about 3 days behind the development of anemia and was com-plete within about 11 days. After recovery from anemia the reciprocal change back to preanemic conditions proceeded at a muchslower rate, HbC

Erythropoietin induces bone marrow and plasma fibroblast

2/3/2017  was barely detectable in Linþ cells. Consistently, human he-matopoietic cells differentiated from umbilical cord stem cells in vitro (Supplementary Figure S1)31 presented the greatest amount of mRNA at burst-forming units erythroid/CFU-E stage (day 3), associated with EPOR mRNA (Figure 1g and h). The amount of FGF23 mRNA decreased at day 6

Combined action of c-Kit and erythropoietin on erythroid

erythropoietin addition) before the cells start to prolifer-ate induced by erythropoietin. During the early phase, erythropoietin down-regulated c-kit gene expression. These results suggest a mechanism of combined action of c-Kit with erythropoietin on the lineage-restricted erythroid progenitor cells. Key words: c-Kit, erythroid progenitor cells,

Activation transcription GATA-1

human and murine erythroid-specific promoters in which GATAelements are more distally positioned (such as the human 'y-globin and mouse a-globin promoters) are not activated in heterologous cells byexpression ofmammalian GATA-1alone (18). To examine GATA-1 action on the EpoRpromoter, we cotransfected EpoR-GH and GATA-1 cDNA expression

Control of Adult Bone Marrow Erythroid Progenitor Cell

progenitors at the colony forming unit-erythroid (CFU-E) stage are responsive to both stem cell factor (SCF) and erythropoietin (EPO); however, the joint action of SCF and EPO in these cells and the underlying mechanisms remain to be defined. In this study, quantitative data on the

Effects of Recombinant Human Tumor Necrosis Factor a

The growth of multipotential, erythroid, and granulocyte-macrophage progenitor cells is influenced in vitro by specific stimulating and suppressing molecules (1-3). Some of the mol ecules implicated in the growth regulation of human hemato-poietic progenitor cells in vitro include PPO4 (4), PPO-a (5), Received 3/31/86; accepted 5/16/86.

Macrophages as novel target cells for erythropoietin

The finding that erythropoietin has effects on dendritic cells thus led to the question of whether macrophages act as target cells for erythropoietin. Design and Methods The effects of erythropoietin on macrophages were investigated both in-vivo and in-vitro. The in-vivo studies were performed on splenic macrophages and inflammatory peritoneal

Signal transduction in erythropoiesis

factors, interleukin 3 (IL 3)2 and erythropoietin (Ep). The most primitive progenitor, the burst-forming unit-erythroid (BFU-E), is characterized by an absolute requirement for IL 3 for continued growth. That these cells are also respon-sive to Ep is shown by the formation in vitro of large clusters (bursts) of as many as 10 hemoglobinized cells in response