Scanning Electron Microscopy Of Red Cell Agglutination

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Comparison of human RNase 3 and RNase 7 bactericidal action

displays a specific Escherichia coli cell agglutination activity, which is not shared by RNase 7. The RNase 3 agglutination process precedes the bacte-rial death and lysis event. In turn, RNase 7 can trigger the release of bacterial cell content without inducing any cell aggregation process. We

l & Bioche Journal of Microbial Biochemical Technology

showed photographs of scanning electron microscope (SEM) before and after treatment of 90% saturated fraction of the lectins extracted from the Egyptian Shalatine cv. seeds against Staphylococcus aureus and Pseudomonas aeruginosa, where a potential agglutination of bacterial cells was shown. *Corresponding author:

Iranian Polymer Journal Archive of SID 15 (8), 2006, 675-683

the inhibition of agglutination by blood-type specific antisera was measured by flow cytometric analysis of fluorescein-labeled anti-D binding to PEG-coated RBCs. Finally, the morphology of PEG-RBCs was studied by scanning electron microscopy (SEM) technique. EXPERIMENTAL Materials mPEG of 2 kDa molecular mass, triethanolamine (TEA)


microscopy: method 43 description 43 iv) The cells viewed in the scanning electron 53 microscope: method 53 description 54 v) Discussion 70 Section 4. The Reactions of the cell-free Haemolymph in vitro 77 i) Introduction 77 ii) The interaction of the haemolymph with 82 vertebrate red cells in vitro: method 82 results 83

Materials and methods. SIMTI Servizi Srl

Scanning electron microscopy. The effect of the conversion process on the morphology of the RBC was investigated by examining the native and enzyme-converted RBC by scanning electron microscopy. The samples were fixed with 3% glutaraldehyde in PBS, dried on a glass slide, and post-fixed with 1% osmium tetroxide in 0.1 M Na-cacodylate for 90

A comparative study of human cell lines derived from patients

The membrane properties of different cultured lymphoid cell lines were stud- ied using Concanavalin A (Con A) cap-forming ability and agglutinability and scanning electron microscopy (SEM) as probes. In addition, enzyme content and ultrastructural features were characterized by cytochemistry and transmis- sion electron microscopy.

Deleterious effects of

Electron Microscopy We mixed 500 mL of whole human blood (three samples for each partic-ulate steroid) with the different steroid drugs and prepared it for transmission and scanning with electron microscopy (Appendix E1 [online]). Statistical Analysis All results were reported as median and quartiles with the number of mice,

HEALTH AND MEDICINE Copyright © 2020 Surface-anchored

analyzed by scanning electron microscopy (SEM) (Fig. 2, D and G). The surface engineering was successful, and an approximately 250-nm-thick apparent gel layer was observed on the engineered cell surface by transmission electron microscopy (TEM) (Fig. 2H) and confocal laser scanning microscopy (CLSM) (Fig. 2I). Conversely, the

Enteroaggregative Escherichia Strains To Adhere In to Human

Mucosal adhesion was assessed by scanning electron mi-croscopy (13). Electron microscopy. Strains were monitored for fimbria production by negative-staining electron microscopy (11). Biopsies with adherent bacteria were processed for trans-mission electron microscopybystandard methodsas previ-ously described (11). RESULTS

An Impedance Aptasensor with Microfluidic Chips for Specific

Scanning electron microscopy anti-N1 antibody and chicken red blood cell (RBC) labels for amplification [34]. is de fined as the agglutination of chicken red

Screening of Lectins for Specific Detection of Listeria

negative bacteria other than L. monocytogenes, but, no agglutination was observed except Salmonella (10-20%), Klebsiella (10-20%), and Citrobacter (45%). The HA of WGA with L. monocytogenes was observed under scanning electron microscopy having cell aggregation with gapped and patchier cells, but, in some cases cell rupture was also seen.

NANO EXPRESS Open Access Structural damage of chicken red

The cell membranes were damaged, the shape of cells was deformed and cells lost their biconcavity. Observation also showed an increasing level of swollen cells as a result of membrane damage after osmotic pressure on red blood cells (Figure 2). Exposure of RBC to NP-Pt and cisplatin caused haemagglutination. Scanning electron microscopy

Congenital dyserythropoietic anaemia type (HEMPAS): a family

outlined for patients with thalassaemia.7 Red cell survival and sequestration studies usingNa251CrO4 (sodium chromate) were performed using standard techniques.8 9 SCANNING ELECTRON MICROSCOPY (SEM) Venous blood was taken without an anticoagulant and added dropwise immediately to a large volume 11'98 copyright. on March 22, 2021 by guest

Chemical camouflage of antigenic determinants: Stealth

scanning electron microscopy (SEM). For the SEM studies, mPEG-modified and control RBC (exposed to pH 9.2 in the absence of reactive mPEG) were prepared as described above, and immediately washed three times in isotonic saline. The cells were then fixed with 1% formaldehydey1% paraformal-dehyde and prepared using standard SEM procedures (14).

Zeodration : a new method for the preservation of blood

agglutination and aggregation were measured in a Carat TX4® aggregometer (Entec GmbH) Platelet adhesion under flow: Fresh (F plt )and zeodrated platelet Z plt suspension were mixed with hirudinated red cell concentrates (v/v) and perfused into a polydimethylsyloxane (PDMS) flow chamber coated with vWF or collagen.

Yeast Cell Architecture and Functions - Wiley-VCH

can only be obtained with the aid of electron microscopy, which in scanning procedures is useful for studying cell topology, while ultrathin sections are essential in transmis-sion electron microscopy to visualize intracellular fine struc-ture (Streiblova, 1988). Atomic force microscopy can be applied to uncoated, unfixed cells for imaging

Iranian Polymer Journal 15 Surface Treatment of Red Blood

morphology of red blood cells was determined by scanning electron microscopy (SEM) technique. Statistical analysis of experimental design together with SEM results showed that the optimum conditions were pH = 8.7, T=14°C, and t = 60 min while the suitable polymer concentration was found to be 12 mg/mL. INTRODUCTION


induced agglutination was determined and correlated with lectin-induced changes in the surface morphology of these cells as studie ind a scanning electron microscope. Whenever the lectin induced high agglutinability in a cell type it also, invariably had a smoothing effect on the cell surface.


sion electron microscopy. The application of latex particles for the detection and localization of cell surface molecules by scanning electron microscopy constitutes a relatively new approach (20). Polystyrene latex particles, 0.2 um in diameter, have recently been used as immunochemical mark-

In vitro Connection Between Biofilm Formation and Virulence

by the agglutination of bacteria in high salt concentrations, from 2.0 to 3.0M. Bacterial Adhesion to Hydrocarbons. Microbial adhesion to hydrocarbon was assessed with xylene according to (Rosenberg and Gutnick, 1980). The degree of adhesion was calculated as [1-(A1/A0)] 100 [%]. Detection of Biofilm by Scanning Electron Microscopy (SEM)


3.3.12 Safranin O staining and scanning electron microscopy 4.4.3 Cell-cell agglutination decreases with extracellular of agglutination in the presence of

Preservation hemostatic structural lyophilized Potential

removed and processed for scanning electron microscopy to visualize platelet adhesion. Citrated blood, platelet-rich plasma, platelet-free plasma (PFP), and red blood cell frac-tions were isolated as described (34). Adhesion of fresh platelets was determined after passing whole citrated blood over the subendothelium. Adhesion of RLplatelets was de-


numbers of pronase-treate and d control cell tso make 0-2 m5l packed cells were then diluted with 5 ml of phosphate-buffered saline (PBS fo)r the Con A experiments. Cell agglutination and haemolysis experiments In order to quantitate cell agglutination and haemolysis, cells treated as described above were placed in 10-ml conical centrifuge tubes.

Microstructure and Physicochemical Properties of Pericardium

of ASD and VSD. Samples for scanning electron microscopy (SEM) Received: April 16, 2019 Published: April 22, 2019 Citation: Gaidash A, Kulak A, Krut ko V, Musskaya O, Kulchitsky V, et al. Mi-crostructure and Physicochemical Properties of Pericardium in Con-genital Septal Heart Defects. Biomed J Sci & Tech Res 17(2)-2019. BJSTR. MS.ID.002980.

Simultaneous Camouflage of Major and Minor Antigens on Red

Materials and Methods: The degree of RBC agglutination by antibodies against the major and minor blood groups was used as a surrogate measurement for quantitative assessment of the effectiveness of the surface coating. Also, the RBC morphology was assessed using scanning electron microscope (SEM).

Cell Surface Properties Correlated with Cohesion in xanthus

Thus, while Congo red treatment blocks agglutination, agglutination is rapidly restored uponremoval ofthe excess Congored (Fig. 1). Electron microscopy. Scanning electron microscopy pro-vides an ideal tool with which to examine the ordering of randomly suspended cells during agglutination. Samples of cell suspensions incubated in agglutination

Mycoplasma gallisepticum-Induced Alterations in Chicken Red

the cells were pelleted and washed in PBS, and the scanning electron microscope. cell pellet was then fixed in 10% buffered formalin. Scanning electron microscopy. The fixed cells RESULTS AND DISCUSSION were prepared for scanning electron microscopy by the Electron Microscopy Service, Department of The morphology of the control RBCs after

Early gametocytes of the malaria parasite P lasmodium

infected red blood cell surface and how do these parasites interact with host cells prior to sequestration. Very limited work has been conducted to investigate this issue, and conclusions from these studies have been conflicting. Two electron microscopy studies reported respectively absence (Sinden, 1982) and presence (Day et al., 1998)

Synthesis and Characterization of Cisplatin-Loaded BSA

transmission electron microscope (TEM) and Fourier transform infrared spectroscopy (FTIR) was done. The effect of particles on RBCs was assayed.Cellular uptake of cisplatin loaded nano-albumin was qualitatively visualized by Confocal laser scanning microscopy.The anti-cancer efficacy of the cisplatin coated albumin

Effect of Spinach Cultivar and Bacterial Adherence Factors on

laser scanning confocal microscopy. Leaf morphology (blade roughness and stoma density) was evaluated by low-temperature and variable-pressure scanning electron microscopy. E. coli O157:H7 persistence on spinach was significantly affected by cultivar and strain phenotypic characteristics, specifically, the expression of curli.

Attachment faecium Duodenal Epithelium of the Small

confirmed by scanning electron microscopy whichshowedthe organism colonizing the sur-face oftheintestine in discrete areas (Fig. 1). A network of interconnecting fibrils can be seen betweenthe bacterial cells, andthese probably also extend from the bacterial cell surfaces to the host plasma membrane (Fig. 2). Thepres

Haemostatic chitosan coated gauze: in vitro interaction with

Scanning electron microscope Scanning electron microscopy was performed using the electron microscope REMMA102 (SELMI, Ukraine) to obtain information about chitosan distribution on cot-ton surface. To avoid surface charge accumulation in the electron-probe experiment standard cotton dressing and gauze-chitosan dressing were covered with the thin

Surface Coating of Red Blood Cells with Monomethoxy poly

control (uncoated) RBCs. The morphology of RBCs was evaluated by scanning electron microscopy (SEM). The effect of polymer coating, based on the immunological response of RBCs, using two kinds of activated mPEG, at optimum conditions of PEGylation was compared. It was

Interleukin‐12 and its procoagulant effect on erythrocytes

We used thromboelastography, scanning electron microscopy, refractome-tery and wide-field microscopy. Our results show that IL12 caused hyperco-agulation, platelet activation and spreading, as well as RBC agglutination. This phenomenon has far-reaching implications for treatment of the

Microscopy, Staining, and Classification

Microscopy Electron Microscopy Light microscopes cannot resolve structures closer than 200 nm Electron microscopes have greater resolving power and magnification Magnifies objects 10,000X to 100,000X Detailed views of bacteria, viruses, internal cellular structures, molecules, and large atoms Two types

Phyto75n04 458 - APSnet

For observation of cell adherence to the hyphae of S. rolfsii, the fungus was grown on water agar plates for 4 days and a suspension of bacteria was poured over the culture. Attachment of cells to the mycelium was observed under light and scanning electron microscopes. All experiments were repeated at least three times. Spore agglutination.

Artificial Spores: Immunoprotective Nanocoating of Red Blood

The confocal laser-scanning microscopy (CLSM) images clearly showed red-fluorescent rings only for [email protected][FeIII-TA]. When we treated RBCs only with TA, we did not observe any fluorescence (Figure S3). We characterized [email protected][FeIII-TA] by Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force

Transmission-Scanning Electron Microscopic Observationsof

stabilized slime appeared as a thick, electron-opaque layer juxtaposed to the outermembrane.Negativestainingandheavy metal shadow-casting revealed an interwoven network of fibrils approximately 4 nm in diameter.

The effect of the neurotoxin lipopolysaccharide on the

bring about inflammatory changes seen in chronic inflammatory diseases, by changing red blood cells (RBCs) ultrastructure affecting coagulations. The following objectives direct the study: to investigate the effects of LPS on the ultrastructure plasma (fibrin networks) and RBCs, using scanning electron microscope (SEM)

Phenotypic variation associated with an anti- phagocytic

Scanning electron microscopy. The scanning electron micrographs of Enterococcus serioli- cida KG+ and KG- phenotypes are shown in Fig. 1 The cell surface morphology of the KG+ phenotype was smooth, while KG- cells had a rough surface. Serum agglutination. Enterococcus seriolicida KG+ was agglutinated with both rabbit anti-KG+