How Are Carbohydrate Concentrations Determined In Cell Biolabs
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Preclinical Evaluation of Safety of Fucoidan Extracts From
ovarian cancer cell line was obtained from Cell Biolabs, Inc (San Diego, CA). The SiHa squamous cell cervical carci-noma, HeLa cervical adenocarcinoma, and the MCF-7 breast cancer cell lines were propagated in a media consist-ing of EMEM. The SKOV 3-GFP-Luc ovarian adenocarci-noma cell line was propagated with media consisting of McCoy s 5a
Aging results in a decline in cellular energy metabolism in
bated at 37 °C for 10 min. Protein concentrations were determined using a protein assay reagent (500-0006, Bio-Rad Laboratories). NEFA con-centrations were measured at 550 nm and 37 °C using a UV/VIS spectrophotometer (SpectraMax M2, Molecular Devices) and standardized to the background and are expressed as μmol mg−1 of protein.
Interleukin-2 Carbohydrate Recognition Modulates CTLL-2 Cell
Although research on the carbohydrate recognition of IL-2 has a long history, its physiological function has not been clearly determined. Sherblom et al. (11) and Zanetta et al. (12) reported that IL-2 recognizes high-mannose type glycans with five or six mannosyl residues as determined by the plate method. Later, Najjam et al. (13) found that
Effect of alternan versus chitosan on the biological
carbohydrate-based scaﬀolding,1 drugorcell delivery,2 5 vaccine adjuvants,6 and wound dressing products.7,8 Regarding their role in cell biology, in addition to carbohydrates serving as a source of energy and a component of cell structure, carbohy-drates also play a role in host immune response by increasing
Characterization of N-Acetylglucosamine Biosynthesis in
concentrations determined. For the N-acetylglucosamine cell wall incorporation assays, 25 µl reaction mixtures were assayed in triplicate with 40 µg Pc protein in 100 mM HEPES (pH 7.8), 1mM MgCl 2, and 1mM UDP-GlcNAc and were placed at various temperatures for 4 h. EDTA/EGTA (50 mM) was added to the assay mixtures to stop the E6
Supplemental Data A Role of DNA-PK for the Metabolic - Cell
Cell, Volume 136 Supplemental Data A Role of DNA-PK for the Metabolic Gene Regulation in Response to Insulin Roger H.F. Wong, Inhwan Chang, Carolyn S.S. Hudak, Suzanne Hyun, Hiu-Yee Kwan, and Hei Sook Sul Supplemental Experimental Procedures Antibodies, Animals, Cell Culture, and Transfection
Glucose and Lipid Metabolism and Oxidative Stress in Estrogen
a Cell Biolabs (San Diego, CA, USA) kit, according to the instructions of the manufacturer. 2.8. Determination of Serum Concentrations of Interleukin 18, Uric Acid, Urea and Creatinine, and Activities of Aspartate Aminotransferase and Alanine Aminotransferase An ELISA kit from Cloud-Clone (Houston, TX, USA) was used to determine the
in vitro - PNAS
determined as the sumofthe carbohydrate content ofboth the Zymolyase-insoluble pellet and the solubilized superna-tant before dialysis. Analysis ofthe carbohydrate retained after dialysis gave the amount of alkali-insoluble (1->6)-f-glucan. Carbohydrate was measuredas hexose (17). (1 6)-3-Glucanwaspurified onalarge scalefor 'IC NMRanalysis
Characterization of Four Outer Membrane Proteins Involved in
with maltose as the sole carbohydrate source (0.3%) to late log phase (optical density at 650 nm of 0.6 to 0.8). The cells were washed once with 20 mM potassium phosphate buffer (pH 7.2) and resuspended in 5 ml of the same buffer. These cells were disrupted by sonication. After the cell extract was separated
RESEARCH ARTICLE Open Access Moderate intensity physical
with a Cell Biolabs Oxiselect™ HNE adduct ELISA kit (Catalog Number STA-338). Nitric Oxide (NO) levels were assessed using a NO (total) Detection Kit (Catalog Number ADI-917-020). Assessments of serum interleukin-1β (IL-1β) concentrations were made through the use of a R&D Systems Quantikine Rat IL-1β/IL-1 F2 Immunoassay
Glucose Assay Kit (Fluorometric) - Cell Biolabs
Cell Biolabs Glucose Assay Kit is a simple fluorometric assay that measures the amount of total glucose present in foods or biological samples in a 96-well microtiter plate format. Each kit provides
Supplemental Information The Transcription Factor - cell.com
algorithm. The 3D positions of T cell nuclear centers for MP-IVM time-lapse recordings were determined in Imaris, xyt coordinates exported and read into an ImageJ script that generated radial profile plots of tracked cells in both maximum intensity-projected green and red channels, using nuclear centers as starting points.
RESEARCH ARTICLE The interaction proﬁle of homologous
Identical concentrations of BSA and trypsinized MmRAD51D were used as negative controls for the DNA binding assays. 2.5 Co-precipitation and Western analysis of proteins from MEF extracts Wild-type MEF, Rad51d /Trp53 MEF (MEF168) and Rad51d / Trp53 / HA-MmRAD51d MEF (MEF172AG) cell lines were previously described . Whole cell extract was
Branched Chain Amino Acid Assay Kit - Meditecno
WARRANTY OF FITNESS FOR PARTICULAR PURPOSE. CELL BIOLABS s sole obligation and purchaser s exclusive remedy for breach of this warranty shall be, at the option of CELL BIOLABS, to repair or replace the products. In no event shall CELL BIOLABS be liable for any proximate, incidental or consequential damages in connection with the products.
Disturbed processing of the carbohydrate‐binding module of
the S-layer of bacterial cell wall . Recently, the 3D structure of the multimodular chitinase ChiW bearing a CBM54 has been determined. Structurally, the CBM54 of ChiW is a right-handed b-helix similar to that of polysaccharide lyases . No clefts typical of type B CBMs or pockets typical of type C ones have been revealed.
Intracellular Butyryl Phosphate and Acetyl Phosphate
the intracellular concentrations of BuP and AcP over the course of fermentations for several C. acetobutylicum strains. We also examined if there is a correlation between BuP and AcP concentrations and the pattern of gene expression as measured by DNA microarrays. MATERIALS AND METHODS Bacterial strains and plasmids.
Novel Carbohydrate Binding Site Recognizing Blood Group A and
cell surface. LT type I B-subunits originating from porcine and human isolates of enterotoxigenic E. coli (pLTB and hLTB, respectively) share 96% sequence identity with each other, but only approximately 80% with CTB (3). Despite great similarity between the carbohydrate binding sites of the B-subunits, evidenced by recent crystal complexes
Identification and Functional Verification of Archaeal-Type
Central Carbohydrate Metabolism (New England Biolabs, Boston, Mass.). The resulting plasmid, Metal ion dependence of atPEPC activity was determined by adding different concentrations (0 to
Research Paper Therapeutic Effects of Targeted PPARɣ
cell formation, RAW 264.7 cells were incubated with increasing concentrations of lobeglitazone, dose-equivalent MMR-Lobe, MMR vehicle, or saline. After stimulation with low-density lipoprotein (LDL, 100 µg/mL) and LPS (200 ng/mL)quantitati, on of Oil Red O (ORO) stained areas was performed by histomorphometirc analysis with Image J software.
Lactate Assay Kit (Colorimetric) - Meditecno
Sample lactate concentrations are determined by comparison with a known lactate standard. The kit has a detection sensitivity limit of 1.5 µM L-Lactate. Cell Biolabs Lactate Assay Kit measures lactate within biological samples.
Metabolic Health Outcomes Following Nine Months of Mild
eners or refined foods are allowed resulting in carbohydrate sources that are complex with low glycemic indices. By default, this plan has an abundance of dietary fiber and plant-derived fatty acids, and rela-tively high concentrations of antioxidants. While altering the diet composition in a way similar to that de-
THE J B C © 2002 by The American Society for Biochemistry and
coconjugates at the cell surface, in the extracellular matrix, and in serum can lead to intercellular adhesion and signaling as well as uptake and degradation. Animal lectins are diverse in structure, but they usually contain modular carbohydrate-recognition domains (CRDs)1 (1, 2). The C-type CRDs are the largest and most diverse class of CRDs.
Letters to the Editor Tissue factor puriﬁed from different
vector (Cell Biolabs, San Diego, CA, USA) containing WT TF or TF N11/124/137A cDNA. WT TF and TF N11/124/137A overexpressed in HUVECs and TF from MDA-MB-231 cells were puriﬁed by afﬁnity chromatography with anti-human TF antibody coupled to Afﬁ-Gel-10 agarose . Placental TF was extracted from acetone powder of human
Glycosylation of tissue factor is not essential for its
To determine whether glycosylation of TF modulates its cell surface expression or procoagulant activity, HUVEC were stimulated with TNFa +IL1b for 6 h and simultaneously treated with varying concentrations of tunicamycin, an inhib-itor of the N-linked glycosyl reaction. As shown in Fig. 1(A), treatment of HUVEC with increasing concentrations of
Characterization of Two Novel -Glucosidases from
(prestained protein marker, broad range [6 to 175 kDa]; New England BioLabs, Herefordshire, United Kingdom). Protein concentrations were determined using the Bradford method (7). Biochemical characterizations of Agl1 His and Agl2 His. Determination of the.
Role of the CipA Scaffoldin Protein in Cellulose
based on membership in the Carbohydrate Active Enzyme (CAZy) data-base (28) or presence of a cohesin or dockerin domain as determined by the Pfam database (29) (see Data Set S1 in the supplemental material). Proteins with Pfam domain PF00963 were designated cohesin contain-ing. Proteins with Pfam domain PF00404 (and no cohesin domain)
Journal Acupuncture esearch
used. A lipid peroxidation assay kit (Cell Biolabs, Inc. San Diego, CA, USA) was purchased and used to check the production of malondialdehyde (MDA), which is a compound of polyunsaturated fatty acids. Dulbecco s modified Eagle s medium (DMEM, Invitrogen, Carlsbad, CA, USA) was used for cell culture. Ischemia surgery
Ethanolic Extract of Anti-Melanogenic E ects in B16F10
Sweden), and lipid was calculated according to the standard procedure . Ash was determined after combustion at 550 C for 4 h in a mu e furnace. Carbohydrate was measured by a phenol-sulfuric acid method . 2.3. Cell Culture B16F10 melanoma cells were obtained from American Type Culture Collection (ATCC; Manassas, VA, USA).
G /M cell cycle arrest by an N-acetyl-D-glucosamine specific
carbohydrate moiety of glycoconjugates on cell surface, resulting in cell agglutination and subsequent precipitation in a solution . The specificity of lectins makes them an important tool in glycoprotein purification, identification and glycan analysis . Lectins are ubiquitous in nature, occurring in plants, humans, animals, fungi, bacteria,
Effect of Low-Sugar and High-Dietary Fiber Noodles Diet on
Assay Kit (Cell Biolabs Inc., San Diego, CA, USA)  and our previous report . The reaction was performed in 75 mM phosphate buffer (pH 7.4), and the volume of the final reaction mixture was 200 µL. Antioxidant (25 µL) and fluorescein solution (150 µL) were added to the microplate wells. The reaction mixture was incubated at 37°C for
High mannose-binding Pseudomonas fluorescens lectin (PFL
In vitro cell adhesion assay The effect of PFL on cell adhesion was evaluated using the CytoSelect cell adhesion assay (Cell Biolabs, San Diego, USA). MKN28 cells were treated with 10 μM PFL for 3 h in RPMI-1640 medium with 10 % FBS. The cell suspensions were allowed to attach to an ECM-coated 48-well plate for 90 min at 37 °C. Adherent cells
Response surface optimisation of high antioxidant jelly from
The carbohydrate content was analysed using total carbohydrate assay kit from Cell Biolabs, Inc. (San Diego, USA). The absorbance was read using microplate reader at 490 nm. A standard curve was constructed using glucose standard solution (0, 0.0625, 0.125, 0.25, 0.5, 1, 2, and 4 mM, R2 = 0.9909). The result of total carbohydrate content was
A Protein Engineering Approach Diﬀerentiates the Functional
biological activity because of the lack of cell surface expression. Lectin inhibition analysis suggested that the carbohydrate at Asn196 is unlikely involved in direct ligand binding. Taking this into account, we constructed a stable variant, with triple mutational deglycosylation (N15D,
The Ca 2+ /calmodulin kinase/AMP-activated protein kinase
saline, were stimulated with increasing concentrations of the lectin for 120 sec. When the time-dependence was assessed, platelets were challenged with 10 µg/mL PHA. In other experiments washed platelets (1.0×109 platelets/mL), preincubated at 37°C with saline, 10 µM STO-609, 20 µM LY294002, 20 µM MK2206 or 10 µM Compound C, were
A new versatile microarray-based method for high throughput
aimed at the deconstruction of cell wall-based feedstocks used forbiorefining(10 12).Carbohydrate-degradingand-modify-ing enzymes are also extensively used in the production of paper,textiles,detergents,feed,andfood(13,14).Endogenous carbohydrate-degradingand-modifyingenzymesarecentralto manyplantdevelopmentprocesses.Forexample,GHsareused
DIAMETRIX DOWN‐REGULATES INFLAMMATORY SIGNALING IN OBESE TYPE
more than 80% of the energy produced by the body is derived from carbohydrate metabolism. When this metabolism is severely limited, the cell initiates oxidation of fat reserves for energy production and degrades proteins to amino acids which in turn are converted
Article Effect of Rosmarinic Acid on the Serum Parameters of
using a Cell Biolabs (San Diego, CA, USA) kit, according to the instructions of the manufacturer. 2.8. Determination of Serum Concentrations of Interleukin 18, Uric Acid, Urea and Creatinine, and
THE JOURNAL OF BIOLOGICAL CHEMISTRY © 1999 by The American
ence was reached. Cell debris was removed by centrifugation (4000 3 g for 4 min) and the medium was stored at 280 °C. MBP was isolated following the method previously used for MBP-C (14). For complement assays, protein was isolated from serum-free medium. Two days after cell confluence was reached, the medium from each tissue culture flask
Total Carbohydrate Assay Kit - Cell Biolabs
standards and unknown samples. Sample carbohydrate concentrations are determined by comparison with a known glucose standard. The kit has a detection sensitivity limit of 62.5 µM glucose. Assay Principle Cell Biolabs Total Carbohydrate Assay Kit measures total carbohydrate within food samples, urine, serum,
A novel family of carbohydrate-binding modules identiﬁed with
alone. The evidence supports a distinctive carbohydrate-binding role of broad speciﬁcity for this type of domain, and we propose a novel family (designated family 37) of carbohydrate-binding modules that appear to be peculiar to R. albus. 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.