Expression Of Growth Regulatory Genes In A SCID Mouse–human Model Of Intestinal Epithelial Regeneration

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Liver stem cells and model systems for liver repopulation

by DA Shafritz 2002 Cited by 150 cardiac mesenchyme in inducing albumin gene expression. [22]. However, other factors or Fig. 1. Schematic diagram of fetal liver development in the mouse.

Physiological importance of various NFκB family members in

by AF Hanedi 2013 Cited by 1 1.6.5 Animal models of colitis associated colorectal cancer 2.2.2 Crypt regeneration assay regulating intestinal epithelial apoptosis and proliferation 4.5 Intestinal expression of cytokine-encoding genes in DSS-induced colitis in null mice showed an increase in AOM/DSS induced colonic tumours.

Cancer stem cell markers in common cancers - DiVA

by T Klonisch Cited by 437 repair make stem cells valuable for regenerative medicine, This coincided with the expression of genes associated with oral epithelium, prostate and urothelia; in prostate: basal prostate cell marker differentiate along a vertical axis within the human gut. In in NOD/SCID mice with morphological and antigenic pro-.

Human tissue-engineered colon forms from postnatal

by ER Barthel 2012 Cited by 24 work, human fetal intestinal cells developed epithelium without mesenchyme following KEYWORDS: animal model ▫ colectomy ▫ colon ▫ humanized mouse ▫ intestinal failure severe combined immunodeficiency (SCID) donor tissue using a regenerative medicine, Expression of growth regulatory genes in a.

Regulation of stem/progenitor cell maintenance by - eLife

by M Tremblay 2020 Cited by 4 natal development, GATA3 is expressed in both basal and luminal cells and Accordingly, Pten-deficient mouse model of prostate cancer exhib- Expression levels of differentially expressed genes between Intestinal epithelium-derived BMP controls stem cell self-renewal in SCID-beige, C57BL/6.

Mechanistic Insights into Colorectal Cancer Phenomics from

30 May 2018 profile of the epithelial cell of origin,4 controls of epithelial Podxl was expressed at the ECM-facing basolateral membrane and underwent directed vesicular transport to the nascent AMIS. For example, regenerating intestinal progenitor of growth regulatory genes in a SCID mouse-human model of in-.

Leonard D. Shultz, Ph.D. Curriculum Vitae - The Jackson

Mouse Model for Investigations of Human Mature Neutrophil Development and Function, The. Jackson Invited Speaker, Humanized SCID mouse models for cancer research, Hollings Cancer Center 2013 Spring CSF-1 regulation of Il6 gene expression by CD34+ cells regenerate gastrointestinal epithelial cells.

Developmental Patterns of Mucin Gene Expression in Human

by MP Buisine 2003 Cited by 6 The lack of a suitable animal model that expresses human intestinal mucin immunodeficient (scid) mice. Expression strated that mucin gene expression in xenografted fetal intestine tal regulation of epithelial proliferation and cytodifferentiation. (18 21). Goblet cell development during xenograft regeneration. (A C) 

Organoids as a model to study the cellular - mediaTUM

by A Pastuła Analysis of genes differentially expressed in the SI organoids from the Establishment of human organoid culture from Barrett´s Esophagus and esophageal Which mechanisms regulate stromal-epithelial cross-talk in the intestinal stem Regulation of crypt growth by the stromal cells in vitro might be mediated by other.

STAT - EMBO Press

by YS Zhang 2019 Cited by 11 intestinal epithelial cells (IECs) in a mouse colitis model. LIF greatly regulated gene expression via STAT4 (Fig EV3A C). Differential analysis 

BCL9/9L-β-catenin Signaling is Associated With - The Lancet

30 Oct 2015 In the intestinal epithelium a gradient of WNT-β-catenin signaling lacking Bcl9/9l proteins were deficient in epithelial regeneration, as Receiver NOD scid models we first assessed expression of the genes that were most strong- Human homologs of common mouse genes were selected according 

Isolation and long-term expansion of a single adult human

23 Jan 2020 renal epithelial cell with efficient kidney regeneration enrichment of genes linked to kidney development and cell proliferation in SCID mice. in the mouse kidneys regardless of the injury model used (IRI, UUO or UPN). SOX9 is an intestine crypt transcription factor, is regulated by the Wnt pathway,.

In vivo restoration of laminin 5 3 expression and - PNAS

by PB Robbins 2001 Cited by 140 with a retroviral vector encoding 3 and used to regenerate human skin on severe combined immunodeficient (SCID) mice. Tissue regenerated from 

Epithelia by B Lymphocytes Homeostatic Regulation of

by Y Nishiyama 2002 Cited by 26 and biological significance underlying this rapid regeneration remain elusive. In this study as well as SCID mutant mice was associated with a marked acceleration of epithelial cell turnover and an up-regulation of the expression of MHC class II molecules. Innumerable intestinal epithelial cells (IEC)3 covering the en-.

Sox17 promotes differentiation in mouse embryonic stem cells

by KK Niakan 2010 Cited by 276 a transcriptional regulator of differentiation in these pluripotent cells. We show these effects of Sox17 on ES cell gene expression are mediated at least in Mouse and human blastocysts tissues support the continued development of epiblast Laboratory for Regenerative Medicine, University of Cambridge, Cambridge.

Role of EPHA3 in colorectal cancer - Tesis Doctorals en Xarxa

by E Andretta 2.1 SELF-RENEWAL IN THE INTESTINAL EPITHELIUM. 4.2 MULTISTEP GENETIC MODEL OF COLORECTAL CANCER 49. FIG.18. REGULATION OF EPH-EPHRIN SIGNALING TO DETERMINE REPULSION OR ADHESION. mouse, expression of the two ligands Sonic hedgehog (SHH) and Indian hedgehog (IHH).

Review The role of Krьppel-like factors in the - Digitum

by MO Nandan 2009 Cited by 81 regulating somatic cell reprogramming. establishment and self-renewal of iPS cells. regulatory sequences of target genes (McConnell et al., their involvement in embryonic stem cell development expressed in epithelial tissues including the gut and skin from ApcMin/+ mice, a model of intestinal tumorigenesis.

Functional intestinal stem cell model - Edinburgh Research

by EM Slorach 1999 Cited by 76 A mouse model of intestinal stem cell function and regeneration regulation of this multipotent population of cells, despite their epithelial development, morphogenesis and differentiation. 1Molecular Genetics Section, MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, UK.

In vivo genome editing and organoid transplantation models

by J Roper 2017 Cited by 152 Δ;KrasG12D/+;Trp53Δ/ Δ (AKP) mouse colon organoids and human CRC the relevant tissue layer for CRC development) or into the mucosa via rectal enema14, resulted in tumors that expressed GFP, indicating stable lentivirus integration. GFP epithelial cells and CRISPR-mediated Apc editing only in Cas9-positive 

Development of Intestinal Organoids from Embryonic Stem Cells

by L Cao 2013 and the analysis of genes expression in cells that make up the IOs. We also In the stem cell zone model, CBCs are characterized as radiation- signaling promotes human and murine HSCs self-renewal, proliferation of NOD/SCID mice, can differentiate into neural and intestinal epithelial cells 19.

The Development of Spasmolytic Polypeptide/TFF2 - CORE

by AC Engevik 2016 Cited by 59 transplantation mouse model promoted gastric regeneration. (Cell Mol Gastroenterol Hepatol Keywords: Epithelial Regeneration; Gastric Cancer; Human.

Regeneration in Vertebrates - ScienceDirect.com

by PA Tsonis 2000 Cited by 257 this review the different types of regeneration in vertebrates and their basic characteristics are presented. The major cellular Expression of growth regulatory genes in a SCID mouse 283 human model of intestinal epithelial regeneration.

Isolation and Propagation of a Human CD133- Colon Tumor

by N Navarro-Alvarez 2010 Cited by 39 able to initiate tumor growth, defined as cancer stem cells (CSC). NANK in NOD/SCID mice induced tumors with developing progressive and CD44, self-renewal genes Oct-4 and Nanog, but showed the expression of an These results provide a novel and extensive model in human CSC for testinal epithelial cells.

ROLE OF EPITHELIUM-SPECIFIC ETS - TSpace

by J Oliver factor-3 (Elf3) in mice, is strongly expressed in lung during fetal development and 1.1.1.2 ESE-1 in regulating epithelial cell differentiation during intestinal 1.5 Airway epithelial regeneration following naphthalene-induced Clara cell injury ESE-1 gene and is 89% identical to its human homolog at the amino acid level 

Unique Gene Expression Signatures in the Intestinal - MDPI

by L Janeckova 2019 Cited by 5 In addition, we analyzed gene expression in colon organoids genes in the mucosa or organoids obtained from GF and CR mice, microbiome, outnumbers the amount of cells present in the human that self-renews and differentiates, generating all main cell types present in the intestinal epithelium.

Dual effects of human adipose tissue-derived mesenchymal

by JJ Rhyu 2015 Cited by 4 by hATMSCs in the xenograft models, global gene expres- sion profiles of neuropeptide Y receptor Y4 were found to be expressed in the ability of self-renewal, proliferation and differentiation the process of carcinogenesis, tumor growth and metastasis, deficient (SCID) and nude mice can be used as human tumor.

Progress and applications of mouse models for human lung

by S de Seranno 2010 Cited by 41 sophisticated somatic mouse models for nonsmall cell lung cancer, small cell a growing number of lung cancer-related genes. epithelial cells: surfactant protein-C promoter directs expression with Hras, can induce lung tumorigenesis and Kras regulatory colon, pancreatic and hepatic carcinomas, melanoma and.

Innovations, challenges, and minimal information for

by R Stripecke 2020 Cited by 24 Abstract. Mice xenotransplanted with human cells and/or expressing Figure 1. Development and applications of humanized mouse models.

The CIEA NOG mouse - Taconic Biosciences

mutation of the Il2rg gene on the. NOD/ShiJic of human cells and tissues in this model Regeneration of damaged tissues. Development of new human cell and Defect of hemolytic complement activity in sera of NOD scid and NOG mice. NODE. EPITHELIAL TISSUES. Large intestine. 48. 0. 14. 17. 0. 4. Stomach. 18.

Differential mucosal gene expression modulates the

by Z Liu 2008 Two animal models of intestinal inflammation used in this dissertation Analysis of mRNA expression of selected genes in the colon of mice treated These results suggest genetic factors are involved in the development of IBD. by ulcers, crypt abscesses, and regeneration of epithelium, but not thickening of the intestinal.

Tissue Recombination Models for the Study of Epithelial Cancer

by Y Zong 2015 Cited by 3 the dissociated cell in vivo prostate regeneration model to investigate prostate cancer. in transplantation-based models of human epithelial cancer. We will The functions of several genes associated with breast cancer development, such as myc implanted under the kidney capsules of SCID male mice (see Protocol: 

BASIC ALIMENTARY TRACT

by J Bassaganya-Riera 2004 Cited by 449 PPAR in the colon were performed in mice by using the Cre-lox immunoregulatory cytokine transforming growth factor. 1 (TGF-1). macologic stimuli into changes in gene expression.4 Each receptor is crypt hyperplasia without regeneration, and epithelial The DSS colitis model resembles human UC because it.

RegIII proteins as gatekeepers of the intestinal epithelium

by LMP Loonen 2013 Cited by 1 ines as well as regulation of production of components of the gut barrier such as anti- lipid A anchor of lipopolysaccharide (LPS).122 A RegIIIβ-/- mouse model was developed Influences of microbiota on intestinal immune system development. expression of HIP/PAP and regenerating gene III in human inflammatory 

Aberrant Epithelial Mesenchymal Hedgehog Signaling

by DH Wang 2010 Cited by 173 imens, human esophageal cell lines, and mouse esophagi. Human induces expression of SOX9, an intestinal crypt transcrip- tion factor, which is tion of gastric columnar epithelium.6 Multiple genetic lium appears as Hh signaling is down-regulated.12 We in several injury repair/tissue regeneration models.15 17.

Expression of growth regulatory genes in a SCID mouse

by A Sattar 1999 Cited by 16 SUMMARY. Analysis of human intestinal epithelial regeneration has been limited. This study has used a novel SCID mouse human model to test the hypothesis 

A CRISPR/Cas9-engineered ARID1A-deficient human gastric

by YH Lo Cited by 1 extensive mouse studies, forward genetic human models are Arid1a is indispensable for stem-cell maintenance and self-renewal, as genetic deletion results However, in Cas9-expressing TP53 KO organoids, over 95% of cells were GFP- development, regulation of Wnt signaling and epithelial to 

Current mouse and cell models in prostate cancer research

by X Wu 2013 Cited by 102 Mouse models of prostate cancer (PCa) are critical for understanding the regeneration, indicating that progenitor cells with luminal characteristics can play a with spatial and temporal regulation of candidate gene expression will cell line is a androgen-sensitive human PCa cell line subtypes of prostatic epithelial.

p53-Dependent acinar cell apoptosis triggers epithelial

by CR Scoggins 2000 Cited by 69 In the absence of p53, upregulation of p53 target genes and acinar cell in mice or rats induces expression of multiple growth factors and regulating pancreatic stem cell biology (2, 3). Because Expression of growth regulatory genes in a SCID mouse- human model of intestinal epithelial regeneration. J Pathol 187:.

Charting human development using a multi - Cell Press

by Q Yu Interrogated genetic and culture perturbations of epithelium the past years as a model to study human intestinal development we found that the intestinal master regulator CDX2 is required expression in the epithelium and mesenchyme of each organ and Regeneration of Damaged Epithelium.

Is teratoma formation in stem cell research a characterization

by M Aleckovic 2008 Cited by 52 Keywords: human embryonic stem cells, pluripotency, teratoma. Teratoma severe combined immunodeficiency (SCID) mice, pluripotent. SC tend to form 

Epithelial stem cell culture: modeling human disease and

by Y Yamamoto 2017 Cited by 4 development of novel in vitro strategies for regenerating epithelial tissues and for closely The isolation and long-term expansion of primary cells, with p63, a master regulator of stemness, in stratified stable propagation of human and mouse epithelial basal intestinal organoids of CF patients, the corrected genes.

IMAGING AND ADVANCED TECHNOLOGY - Gastroenterology

by VSW Li 2012 Cited by 32 Mice lacking Tcf7l2/Tcf4, a key transcription which encodes a key negative regulator of the Wnt path- Regeneration of intestinal epithelial cells is fueled epidermal growth factor; RFP, red fluorescent protein; SCID, severe derived from human colon cancer. tablish a long-term in vitro culture model in which 3-di-.

Cancer Stem Cells in Colorectal Cancer

compartment of the intestinal epithelium, every crypt has a monoclonal origin and multipotent pathway probably governs stem cell activation by regulating nuclear localization Gene expression profiling of Lgr5+ epithelial cells isolated mouse models as well as in human CRC highlights the idea that cancer may be.

the applicability of biological models to the understanding of

by J Lin 2011 Cited by 39 intestinal epithelium and host leukocytes to regulate mucosal injury, repair and regeneration; and how alterations in the intestinal microvasculature of the mice and humans (Gosai et al., 2010), as well as for using gene- silencing by histological features of inflammation and necrosis, an increase in the expression of.

Chemical conversion of human epidermal stem cells into

14 Apr 2021 repair of the intestinal epithelia in a colitis mouse model. Thus regulation in the intestine. (C) in the presence of basic fibroblast growth factor (bFGF) and 1Research Center for Tissue Repair and Regeneration affiliated to the Medical Inno- acquire the key gene expression phenotypes of GCs.

Regulatory T-cells regulate neonatal heart regeneration by

by J Li Cited by 36 Treg directly promote proliferation of both mouse and human cardiomyocytes in a paracrine till postnatal day 7 (P7) in a range of injury models neonatal heart regeneration in NOD/SCID mice after significantly increase in cardiac fibrosis (Figure then examined gene expression by qRT-PCR with a.

Gene Signature Based Approach Identified MEK1/2 as a

by K Gamo 2018 Cited by 1 to revert expression of genes dysregulated in infliximab responders. Finally, using murine colitis model, administration of MEK1/2 inhibitor significantly to the promotion of reconstitution of intestinal epithelium. Human Gene Expression Kit. mice were intravenously injected into SCID mice (day 0). At.

Biochemical Journal - University of Alberta

by ZD BURKE 2007 Cited by 48 Key words: cell differentiation, intestine, liver, pancreas, stem announcement that human stem cells could be derived from the Figure 1 Scheme of stem cell self-renewal and differentiation endoderm specification is regulated, in part, by epithelial Expression of Wnt genes during mouse development suggested.

Wnts as Essential Growth Factors for the Adult Small Intestine

by J Hoffman 2004 Cited by 75 expression of Dkk1 resulted in rapid inhibition of Wnt target gene expression and of The profound developmental roles of Wnts suggest equally essential regulatory roles in were severely and equally affected in Ad Dkk1-treated SCID mice Gradient of inhibition of small intestinal epithelial proliferation by Ad Dkk1.

The PTEN Phosphatase Controls Intestinal Epithelial Cell

by MJ Langlois 2010 Cited by 77 the specific cellular role of PTEN in human intestinal epithelial cells. The aim of this during colorectal tumor progression with down-regulation of both proteins being OAS1 gene expression was detected by Q-PCR analysis in the models. Female nude mice CD1 nu/nu and Fox Chase SCID Beige mice.