Functional Analysis Of Rex1 During Preimplantation Development

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Inhibition of β-catenin TCF1 interaction delays

during self-renewal (top 30% NanogHigh from serum plus LIF) with NanogLow cells during differentiation (bottom 30% Nano-gLow from serum plus RA). The data set analyzed for differen-tial expression and functional enrichment (Table S2) confirmed that although NanogLow cells exhibit lower expression of plu-

REVIEW ARTICLE Human Embryonic Stem Cells as a Powerful Tool

Analysis of the contracting areas in the HEB indicates that they have physiologic characteristics sim-ilar to early cardiac tissue (18). Additionally, progressive stages of early cardiomyocyte differentiation are observed during HEB differentiation (19). It is also possible to obtain a variety of functional cardiac cells in vitro (nodal-like

Stem Cell Reports

Rex1-EGFP reporter during preimplantation embryo devel-opment and ESC derivation. We evaluate the general requirement for REX1 in vitro and in vivo, and characterize the utility of the Rex1-EGFP reporter for studying self-renewal and differentiation status of rESC cultures. RESULTS Generation of a Rex1-EGFP Reporter rESC and Rat

YY2 in Mouse Preimplantation Embryos and in Embryonic Stem Cells

May 07, 2021 its paralog YY2 contribute to this regulation during preimplantation development and in mESC has not been extensively studied. Despite the presence of both YY1 and REX1 in preimplantation embryos [1,13], neither the presence of YY2 at preimplantation stages nor a functional role for Yy2 at stages

l-Proline as a modulator of ectodermal differentiation in ES

Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture. Theriogenology 49: 83 102, 1998. 11. Gingras AC, Raught B, Sonenberg N. Regulation of translation initia-tion by FRAP/mTOR. Genes Dev 15: 807 826, 2001. 12. Gurdon JB, Melton DA. Nuclear

A Protein Roadmap to Pluripotency and Faithful Reprogramming

cently, using an integrated functional genomics approach, Ivanova et al. [2006] have demonstrated that Esrrb, Tbx3 and Tcl1, as well as previously identified Nanog, Oct4an d Sox2 , are required for efficient self-renewal of ES cells in vitro. Downregulation of each gene induces differentia-tion of ES cells along specific lineages.

Taking the RISC of exiting naïve pluripotency

(Fig. 1). Studies of the functional inhibition of DNMT, KDM, and SWI/SNF during the ESC-to-ELA transition showed that the activity of these chromatin regulators during priming is necessary for down-regulating both the naïve marker Nanog and markers of pluripotency (Klf4, Rex1, and Dax1) and for up-regulating priming markers [1].

A transcriptome-wide antitermination mechanism sustaining

RNA-sequencing (RNA-Seq) analysis uncovered considerable changes in the transcriptome of siSrrt-treated ESCs with 1828 downregulated and 1590 upregulated genes [FC≥1.5 and false discovery rate (FDR)<0.05; Supplementary Data 2]. The regulated genes showed a partial overlap with those changing their expression during spontaneous differentiation

The Lectin Dolichos Biflorus Agglutinin Recognizes Glycan

marker transcripts such as Rex1, Gbx2, and c-myc [3, 5, 7] have been reliable readouts for the pluripotent state and early differ-entiation. Their utility is limited, however, because these mark-ers cannot be used for the analysis and recovery of functional cells and they have severe limitations at the single-cell level of analysis.

Cell Stem Cell Article

Cell Stem Cell Article Prediction and Testing of Novel Transcriptional Networks Regulating Embryonic Stem Cell Self-Renewal and Commitment Emily Walker,1 Minako Ohishi,1 Ryan E. Davey,1 Wen Zhang,2 Paul A. Cassar,3 Tetsuya S. Tanaka,1

Preliminary programme 16 octubre - SEBD

13- The role of rex1/zfp42 in preimplantation development pertains regulation of endogenous retroviral elements 84 19- Functional analysis of Rex1 during

KLF3 promotes the 8‐cell‐like transcriptional state in

embryo development. The heterogeneity is typically defined by transcriptional ac-tivities, for example, the expression of Nanog or Rex1 mRNA. Our objectives were to identify mESC heterogeneity that are caused by mechanisms other than transcrip - tional control. Materials and methods: Klf3 mRNA and protein were analysed by RT-qPCR, Western

008 - Palmqvist

including Stat3, Rex1, Sox2, Gbx2, and Bmp4. A significant number of the decreased genes also overlap with previously published mouse and human ESC data. Furthermore, sev-eral membrane proteins were among the 48 decreased genes correlating most closely with the functional assays, including the stem cell factor receptor c-Kit.

RESEARCH ARTICLE Open Access Changes in sub-cellular

Background: Preimplantation bovine development is emerging as an attractive experimental model, yet little is known about the mechanisms underlying trophoblast (TE)/inner cell mass (ICM) segregation in cattle. To gain an insight into these processes we have studied protein and mRNA distribution during the crucial stages of bovine development.

Defining the three cell lineages of the human blastocyst

preimplantation development rather than assuming equivalence to the mouse. RESULTS Comparative transcriptomics analysis throughout human and mouse preimplantation development reveals temporal differences in gene expression To unravel similarities and differences between human and mouse embryogenesis, we compared their preimplantation transcriptomes

Research Paper The molecular characterization of porcine egg

determined. Germ cell development is initiated from a small population of precursor cells known as primordial germ cells (PGCs), that initially express Fragilis (Ifitm3) followed by the expression of Blimp1 (or Prdm1) and Stella (or Dppa3) [36, 37], which proliferate and migrate to the genital ridge during early embryo development [38].

Evolution of the mammalian embryonic pluripotency gene

period during which mammalian embryonic cells need to be main-tained in an undetermined state before engaging in early differenti-ation events. Embryonic pluripotency is an essential property of a small group of cells of the mammalian blastocyst which transiently keeps them in an indeterminate, uncommitted state. This condi-