The Dynein Heavy Chain Gene Family In Tetrahymena Thermophila

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I have used the ciliate Tetrahymena thermophila to study PTMs of tubulin as this organism has several experimental advantages to offer. Tetrahymena is easy to grow to high density and on a cheap medium with a generation time of only 3 hours. Routine gene manipulations (including gene knockouts) can be carried out that involves incorporating

The Dynein Family at a Glance

T. thermophila Axonema l Outer-arm dynein C. reinhardtii M. musculus DHC5 H. sapiens DHC5 M. musculus DHC8 H. sapiens DHC8 Inner-arm dynein Inner-arm dynein D. melanogaster C. reinhardtii DHC 1 D. melanogaster C. reinhardtii DHC1 0 C. reinhardtii DHC9 H. sapiens DHC7 H. sapiens DHC3 Cytoplasmic III V VI I IV VII II AAA+ Helix 2 insert Dynein

Sequence, Biogenesis, and Function of Diverse Small RNA

Aug 06, 2009 1 Sequence, Biogenesis, and Function of Diverse Small RNA Classes Bound to the Piwi-family Proteins of Tetrahymena thermophila Mary T. Couvillion, Suzanne R. Lee, Brandon Hogstad, Colin D. Malone, Leath A. Tonkin, Ravi

James D. Forney Professor of Biochemistry

Nov 20, 2013 translation elongation factor 2 in Tetrahymena thermophila. Gene 326, 97-105. 11. Ku M, Mayer M and Forney J. 2000. Developmentally regulated excision at a 28 bp sequence from the Paramecium genome requires flanking DNA. Mol. Cell. Biol. 20, 8390-8396. 12. Thai K and Forney JD. 2000. Analysis of the conserved cysteine periodicity of

James D. Forney Professor of Biochemistry

mutations is gene specific in Paramecium tetraurelia. Mol. Cell. Biol. 14, 2479-2484. 26. Asai D, Beckwith SM, Kandl KA, Keating HH, Tjandra H and Forney JD. 1994. The dynein genes of Paramecium tetraurelia: Sequences adjacent to the catalytic p-loop identify cytoplasmic and axonemal heavy chain isoforms. J. of Cell Science 107, 8139-8147. 27.


Centrin, an EF hand Ca2+ binding protein, has been cloned in Tetrahymena thermophila. It is a 167 amino acid protein of 19.4 kDa with a unique N terminal region, coded by a single gene containing an 85 bp intron. It has > 80% homology to other centrins, and high homology to Tetrahymena EF hand proteins calmodulin and TCBP23, 25.

Vol. 10, 771 784, March 1999 Gene Knockouts Reveal Separate

cell that expresses multiple dynein heavy chain genes. Substantial portions of two Tetrahymena cytoplasmic dynein heavy chain genes were cloned, and their motor do-mains were sequenced. Tetrahymena DYH1 encodes the ubiquitous cytoplasmic dynein Dyh1, and DYH2 encodes a second cytoplasmic dynein isoform, Dyh2. The disruption of

Manipulating Dynein Genes in Tetrahymena

Manipulating Dynein Genes 271 Like fungi,Tetrahymena genes can be disrupted or replaced exclusively by homologous recombination. However, unlike fungi, Tetrahymena expresses more than a dozen different dynein heavy chain genes. The number and sequences of the family of dynein heavy chains are conserved across a widely

Microtubule-Nucleus Interactions in Dictyostelium discoideum

the dynein heavy chain and kif13hairpin constructs. For inducible expression, the entire hairpin loop cassette was excised with SalI and MluI digestion and then was blunt/sticky-cloned into BglII/MluI sites in the MB38 plasmid (2). Standard molecular biology procedures were followed for DNA isolation, manipulation, and blotting.

Paper Specific Instructions 1. A B C 2. A Multiple Choice

Q.15 In rearrangement of immunoglobulin heavy chain, N-nucleotides (A) are added by the enzyme terminal deoxynucleotidyl transferase (B) form a palindromic sequence that is added to the end of gene segment (C) form a palindromic sequence that is added to the single stranded end of the coding DNA

Single-Cell Transcriptomics Reveal a Correlation Between

Transcriptomics Reveal a Correlation Between Genome Architecture and Gene Family Evolution in Ciliates (2019). Biological Sciences: Faculty Publications, Smith College, Northampton, MA.