Detection Of Allergens In A Cat Pelt Extract

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Lifestyle and indoor air pollution: allergens

allergens collect in dust reservoirs within the house, and as a result of activities which cause settled dust to become airborne (bed-making, vacuuming), the allergens may be

T cell responses to der f2 mite allergens in Thai allergic

duced by mite allergens. Sera from 24 HDM- allergic patients and 20 non-allergic subjects were measured for IgE reactivity by ELISA. PBMC was cultured with mite allergens (Df, rDerf2) and mitogen (PHA). The supernatants and cell pellet obtained were evaluated for cy- tokine production by ELISA and cytokine gene expression by RT-PCR, respectively.

Chemistry & Biology, Vol. 11, 1361 1372, October, 2004, 2004

Horsham RH12 5AB tivity may be a common mechanism for other allergens, United Kingdom such as cockroach allergen Per a 1 [5], cat pelt allergen 4 Institute of Infection, Immunity and Inflammation Fel d 1 [6], and allergens from rag weed [7]. Proteolytic

Domestic allergens in public places II: dog (Can f 1) and

received less scientific attention than cat allergy, partly because of the impression that dog sensitivity is a relatively minor clinical problem. Dander, pelt hair and saliva are the most important sources of dog allergens, and urine does not exhibit significant allergenic activity [5,6]. A major dog

Allergen Immunotherapy

severity, detection of specific IgE sensitivity to seasonal or perennial allergens may be indicated as a basis for education about the role of allergens for avoidance and for AIT. (p 167) Asthma is a risk factor for fatal anaphylactic reactions to AIT (p 168) The Expert Panel recommends that AIT be considered for

Surface washes of living cats: an improved method of

than whole pelt extracts. The possible advantages of such a preparation for therapy and diagnosis of cat allergy in man are discussed in the light of our present knowledge of cat allergens. METHODS Surface washes of live cats Mature cats (1.5 to 3 kg), shown to be in good health by a

The prevalence of indoor allergens in the Baltimore area

tibodies to Dp 42 and to Dm 6, (2) cat: oligospecific rabbit antibodies to Cat Ag 1, obtained from polyspecific anticat- dander antibodies by immunoabsorption on CNBr-activated Sepharose 4B, coupled with a pooled Sephadex G-75 frac- tion (molecular weight XiO,OOO) of a cat-pelt extract. This

ORIGINAL RESEARCH

(Blattella germanica), cat dander, dog dander, dust mite mix, dust mix, mixed grass pollen, mugwort/sage, ragweed, weed mix, mixed tree pollen, mold mix, Penicillium, Alternaria tenuis, and mouse pelt were applied to the skin of the forearm (Alk-Abello, Round Rock, TX). A test was considered positive if the maximum diameter of the wheal was

Dog and Cat Allergies: Current State of Diagnostic Approaches

component allergens have been identified in serum, dander, pelt, hair, saliva, and urine. Extracts prepared from dog liver, se-rum, salivary glands, and keratinocytes contain fewer IgE bind-ing allergens compared to dander. Initial studies that showed the importance of the dog component allergen Can f 1 suggests

RESEARCH Open Access Allergen-specific T cell quantity in

Allergens Allergen extracts (ALK-Abello, Horsholm, Denmark, except for Timothy grass pollen extract from Greer Laboratories, Lenoir, NC, USA) were kindly donated by Western Allergy, Mississauga, Ontario, Canada. Neat extracts contained 50% glycerol and 0.4% phenol. Nega-tive control was 0.9% sodium chloride in 50% glycerol

Specific allergen concentration of WHO and FDA reference

Cat hair E4 10,000 BAU/mL or 7.0 Fel d 1 units/mL Fel d 1 5.4 mg/mLk Cat pelt E4 10,000 BAU/mL or 14.8 Fel d 1 units/mL Fel d 1 15.6 mg/mLk Cat hair E5 10,000 BAU/mL or 22.0 Fel d 1 units per mL Fel d 1 24.8 mg/mLk BAU, Bioequivalent allergy unit. *Mean of duplicate determinations. Based on these data, 1 IU Can f 1 5;5 ng Can f 1.

A differential effect of 2 probiotics in the prevention of

performed SPTs at 2 years to egg white, peanut, cow s milk, cat pelt, Der-matophagoides pteronyssinus, and mixed grass pollen (Hollister-Stier, Spo-kane, Wash). This panel of allergens has been shown to identify 90% of