IL‐7 And Immobilized Kit‐ligand Stimulate Serum‐ And Stromal Cell‐free Cultures Of Precursor B‐cell Lines And Clones

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MiR221 promotes precursor B‐cell - MPG.PuRe

by G Petkau 2018 Cited by 6 that miR221 regulates bone marrow retention of such B-cell precursors by targeting PTEN, thus enhancing This differentiation is mediated by the cytokine IL-7 and the tran- scription positive clones, tested for PTEN expression, showed an increased immobilized Kit-ligand stimulate serum- and stromal cell-free cultures.

CD40 ligand upregulates expression of the IL-3 - Nature

by M Zhou 2000 Cited by 18 tors for IL-7 (IL-7R) and stem cell factor (SCF-R, c-Kit) was also induced by of BCP-ALL cells cultured in serum-free media with CD40L plus these factors.


While there have been over 400 continuous cell lines from have cloned genes whose regulatory sequences contain enhancers 7 Morphogenesis of a Hymenopteran Cell Line However, high density serum-free culture of insect cells in cytes (CTLs) from Precursor Cells. factor (G-CSF), IL-11 and ligand for c-kit.

B-cell receptor-dependent antigen phagocytosis drives - UAM

1.1 Cell lines B cell phagocytosis of antigens stimulates the humoral response in-vivo Interleukin secretion in cultured germinal centers is RhoG-dependent. 2004), since its ligand (CXCL12) is produced by stromal cells located in the DZ beads with covalently bound antigen, the PolyLink Protein Coupling Kit.


by E Ruuth 1988 Cited by 1 growth factor (BCGF or B cell stimulating factor, BSF), distinct from T cell growth factor arginini mimicking the effects of a BSF on B cells was recognized. hematopoietic stem cell However, LPS stimulation of pre-B cell lines and macrophages results in In the series of recently purified and cloned factors one finds IL-7.


by S Lopez-Lastra 2017 In vivo efficacy of umbilical cord blood stem cell derived NK cells in the treatment of immunobiology with tumors arising from simpler tumor cell lines to the transplantation of different cell precursor origins and culture conditions thus creating Full-length human IL-2, IL-7, IL-25 and IL-33 cDNA clones were cloned in the.

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However, most of these data were obtained in vitro, often after stimulation with non as pre-B I, based on cloned cell lines responding to IL-7 and stromal cell contact.4 Kit ligand is expressed by stromal cells in bone marrow, while c-kit is [3H]TdR incorporation, provided the cells are cultured in serum-free media devoid 

Occasional review Cytokines in asthma - Thorax BMJ

by KF Chung 1999 Cited by 876 eosinophils and eosinophil precursors into the v B cell growth factor; neutrophil chemoattractant; T cell and epithelial stimulating factor; SCF = stem cell factor; IFN = interferon; PDGF = platelet the IL-2 receptor γ-chain (IL-2, IL-4, IL-7 and SCF (c-kit ligand) is produced by bone marrow MIP-1α immobilised by.


by IB Fisher 2004 we analyzed Ets-2 activation in T-cell lines, and created two transgenic mice Calcium stimulated activation of Ets-2 T72A in Jurkat T-cells Hematopoietic stem cells that remain in the bone marrow can develop into B- pre-TCR may not have an extracellular ligand. RBC free serum was collected from the samples.

DNA Methylation Changes in Hematopoietic - RWTH Publications

3.4.3 Distinct DNA Methylation Patterns of Human B Cell Development Human induced pluripotent stem cells (iPSCs) represent an attractive cell and iPS-MSCs in the course of differentiation on days 1, 5, 7, and 35 by phase Stable iPSCs clones were cultured in a feeder-free system in either mTeSR1 medium.

In vitro cell and culture models for osteoblasts and - -ORCA

by EM Czekańska 2014 Cited by 4 human osteoblasts and human bone-derived mesenchymal stem cells (MSCs) were co-cultured cells stimulate in vitro mineralization of MG63 osteoblast precursors. MSCs cytokine gene expression after culture in serum-free medium. 154 of isolating clones from cell lines contradicts the opinion of their homogeneous.

BD Biosciences Stem Cell Source from isolation to analysis

BD Procount™ Progenitor Cell Enumeration Kit Embryonic stem cells can be relatively easily grown in cell culture, while adult stem Binding by fetal calf serum (FCS) Structural filament protein indicative of progenitor cell lines including thymic precursor. T lymphocytes. IL-7. NK cells. Flt3 ligand, IL-7, IL-15, IL-2, 

Clinical abstracts - The Journal of Immunology

Depressed cell-mediated immune responses, B cell hyperplasia and result from changed in presence of HIV peptides: IFN-7, TNF-a, IL-to were increased and 


by L MacLellan 2008 2.2.2 Culture of non-B cell lines Analysis of human bone marrow stromal cells and SMS-SB cells contacts with the IL-7 dependent B cell precursors 25. ligand has been reported to boost proliferation of CD19+/CD34+ pro-B cells 39 promoting to SMS-SB cells cultured in serum-free medium at low 

Basic Cell Culture Protocols Basic Cell Culture Protocols

design features that will facilitate contamination-free culture. If a new cell cul- For primary cells and newly acquired cell lines, it is advisable to culture cells with and Many of the cloned cytokines can stimulate hematopoietic cells in vitro at potentiate B-cell production, including stem cell factor (SCF), Flt-3 ligand (Flt-3L),.

Chapter 3 T-Lymphocytes: Mature Polyclonal and Antigen

that secrete cytokines that enable B-cell differentiation and immunoglobulin. 45 polyclonal and antigen-specific T-cell culture and in vitro measures of Of the pre-made serum free media, X- IL-7, produced by BM stromal cells, supports the growth of T- stimulate T-cell clones and enable autocrine proliferation [ 129].

Production and function of a soluble c-Kit molecule - Adelaide

by SH Read 2001 The receptor tyrosine kinase c-Kit and its ligand Steel Factor (SLF) are important in ELISA assay the culture supematant of several FDC-P1 cell lines expressing various Il'heterozygotes have defects in these areas to varying degrees' The stem cells proliferation pre-B cells (McNiece et at., l99lb; Billips et al., 1992).

UC Riverside - eScholarship

by V Gusti 2014 Intestinal epithelial cells tight junction and TNF receptor/ligand Transferred peritoneal B cells produces serum IgG and IgM but not. IgA when peritoneal cavity after two weeks without antigen stimulation. cells, APC and stromal cells (29,30). mice and cell culture model studies classify the claudin family into two main.

Abstracts - Karger Publishers

serum-free media and PIXY321 The cells were cultured for 12 days with the addition of media at To overcome the low efficiency of gene transfer into hematopoietic stem cells, we have differentiation of CD34+ cells and bipotential myeloid cell lines. growth and proliferation of PU.l null liver or bone marrow cells to IL-3, 

Discovery and characterization of the ligands of NK-cell

by WF Garcia Beltran 2016 Doctoral dissertation, Harvard University, Graduate School of Arts & To ascertain non HLA-I ligands of KIR3DS1, we found that cell lines of various Figure 5.5: KIR3DL1+ NK-cell clones are disinhibited by TUN treatment of 721.221- (b) BCL cells were cultures for 48 h in sulfate-free media containing the indicated 

A thesis submitted in conformity with the requirements

by BL Kee 1995 FCS: fetal calf serum Survival of clonable B cell progenitors in the absence of IL-7. IL-7 stromal cells and stromal cell-derived factors promote B lineage progression Analysis of the Ig loci in A-MuLV transformed pre-B cell lines suggests that kit ligand (KL), and is encoded by Sl (Anderson et al., 1990; Copeland et al., 

Immunobiology Handbook - BioLegend

Cell Vitality Indication through Esterase-dependent Probes 29. Apoptosis Mouse FcR-related non-specific staining caused by B cells, monocytes/.

Download PDF - Experimental Hematology

by E Lee 2014 Cited by 27 blood cells from hematopoietic stem cells via large-scale manufacture is an alternative way of meeting IL-6 and TPO for 7 days, followed by culture for a further.

ILâ '7 and immobilized Kitâ 'ligand stimulate serumâ ' and

by Y Kawano 2017 Cited by 5 and stromal cell-free cultures of precursor B-cell lines cell lines and clones from fetal liver, but are necessary to establish such cell lines from bone marrow. Keywords: IL-7 r Kit-ligand r Pre-B cell r Proliferation r Serum-free r 

RANKL Synthesized by Both Stromal Cells and Cancer Cells

15 Jan 2013 We discriminated the roles of RANKL synthesized by stromal cells medium of HSC3 and HO-1-N-1 cells in a co-culture of murine bone producing RANKL, which had been stimulated by IL-6 for an additional 48 hours in 4 mL of serum-free a-MEM. The CMs derived from these oral cancer cell lines.

Stable lines and clones of long-term proliferating - CORE

by Y Kawano 2018 Cited by 5 CLP clones could be induced to differentiate to T, B, natural killer, dendritic, and our experience with serum-free cultures of pre-BI cells from fetal stimulation by Flt3L and low doses of interleukin-7 (IL-7), in the Plate-immobilized recombinant kit-ligand, which we previously found to be able to replace.

Gender differences in urothelial bladder cancer -

14 May 2021 androgens may promote bladder cancer development directly by and either enhance or depress transcription of multiple genes [7]. Blocking mouse IL-6 mice significantly reduced PCa stem cell numbers [27]. IL-7-stimulated B cell precursors cultured independently of stromal cells did not respond to 

©Ferrata Storti Foundation - Haematologica

by C Bordignon 1999 Cited by 168 donor-derived, virus specific T-cell lines or clones rep- resents a more presence of SCF, IL-7, IL-2 and bone marrow stromal cells producing IL-15. This last 


24 Aug 2013 2013 ISEH - Society for Hematology and Stem Cells. similar to PGE2 can stimulate stem cell migration. subsequently leads to the appearance of the pre-B cell receptor that Kit-signaling regulates the competition between mouse able aggregate sizes to culture PSCs towards HPCs in serum-free 

The molecular mechanism of the aberrant expression of the B

by D Ray 2013 binding pattern in B cells, t(8;21) AML and non t(8;21) myeloid precursors 152 3.10 siRNA mediated knock-down of c-KIT down-regulates PAX5 expression Table 2.1: Culture Condition used to grow human cell lines 102 that IL-7 and FLT3 signalling 'instructs' the B-cell specific transcription factors,.

Effects of nerve growth factor on rat peritoneal mast cells

by K Horigome 1994 Cited by 181 period of 2-6 days in conventional serum-containing me- dium. cell(s); SCF, stem cell factor; IL, interleukin; NGF, nerve growth factor; Pre-eminent among these is stem factor, or c-kit ligand. IL-3-dependent mast cell lines are transfected with growth fac- NGF appears to promote the survival of primary cultures of.

Science Journals AAAS - Science Signaling

29 Nov 2016 The pre B cell receptor (pre-BCR) is an immature form of the BCR critical for sulted in the formation of large, highly immobile pre-BCR aggregates, which was mous signaling is driven by ligand-independent self-association of the ress from dependency on interleukin-7 (IL-7) to the stromal presen-.

Growth Factors VEGF-A165 and FGF-2 as - MDPI

by A Sedlář 2021 stimulated proliferation only in HUVECs, while FGF-2 stimulated the fibroblast growth factor (bFGF); adult stem cells; endothelial cells; cell worth mentioning that VEGF-A165 immobilized on cell cultivation substrates can also act as cells grown in matrigel with fully defined serum-free culture media.

Dr. Anita Sapoznikov - Weizmann Institute of Science

by A Sapoznikov 2009 Macrophage/Dendritic cell Precursors 2.2.3 DC ablation causes depletion of recirculating B cells from BM antigen stimulation, which fulfill the function of BM as a secondary lymphoid organ. express CD117 (c-kit, the receptor for stem cell factor), but no mature lineage contact with IL-7-producing stromal cells [59].


by H Guven 2005 Gene transfer into NK cell lines. CD34+CD7+ cells could generate NK cells when cultured in IL-2 alone (Miller acting cytokines such as stem cell factor (SCF) also known as c-kit ligand, further differentiate to give rise to B-, T- and NK-cell-restricted precursors. CellGro SCGM serum-free medium was used in all the.

(IGF-II) Receptor Inhibits Interleukin-6-Type Cytokine

by L Duplomb 2003 Cited by 12 receptor; FCS, fetal calf serum; gp130, glycoprotein 130; IL-6R, IL-6 receptor; LIF hybridoma B9 and 7TD1 cell lines were cultured in RPMI 1640 supple-.

Chapter 1 - The University of Liverpool Repository

by F Al-Tae c-kit. (KL) ligand, flt-3 (FL) ligand and cytokines such as IL-2, IL-7, IL-15 and IL-21 the expression of IL-15 receptor complex on NK cell precursors (Yu et al, 1998). NK cells, i.e. T cells, B-cells, stem cells, dendritic cells, monocytes, granulocytes and The latter is a serum free medium specifically developed for long.


by H Khaja Cited by 2 Epstein-Barr Virus Cell Lines as a Model System for Studies in Activated B Cells 27 Figure 2. Murine B cell cultures stimulated with IL2 and a TLR7 agonist undergo Uric acid rescues TK6 cells from death associated with serum perforin; or tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) [5].

B cell development in fetal liver and adult bone marrow

Cloning and characterization of germline transcripts from light chain variable Comparison of gene expression patterns between B cell precursors derived from fetal Pax5 is induced in response to IL-7 (DeKoter et al., 2002) and there is Pro- and pre-B-I cells can be grown very efficiently in tissue culture on stromal cells 


Therapeutics Research and Development Stem Cell Research such as interleukins IL-2, IL-7, and IL-10, granulocyte colony-stimulating factor (G-CSF), Using the RNAscope™ detection kit and accompanying quality reagents including cell culture media, serum, defined media Cathepsin B (Native Protein).

Heparan sulfate proteoglycans in B cell maturation and

progenitor cell or precursor cell, identified as the hematopoietic stem cell (HSC). Dependent on the strength of the BCR signal the B cell clones will rapidly Figure 7. Schematic overview of the generation of conditional transgenic mice on a BL/6 cell lines to bone marrow stromal cells stimulates interleukin-6 secretion.

Decoupling the Functional Pleiotropy of Stem Cell Factor by

by CCM Ho 2017 Cited by 44 Chia Chi M. Ho,1,4,11 Akanksha Chhabra,3,14 Philipp Starkl,6,7 Stem cell factor (SCF), also known as Kit ligand, mast cell Several lines of evidence suggest that the biological activity of spondingly, stimulation with SCF dimers resulted in the most (B) Overlay of size exclusion chromatograms.

Flow cytometric analysis of growth factor receptor expression

by M de Jong 1997 7. Coexpression of Kit and the receptors for erythropoietin, interleukin 5 and stem cell factor (~KL, Kit ligand; MGF, mast cell growth factor; SF, characterization on hematopoietic cells and small cell lOng cancer cell lines. serum-free cultures stimulated with purified recombinant granulocyte colony-stimulating factor.

Modulation of the haematopoietic stem cell niche by - RUN

by ENOQ Costa 2018 Controlling the differentiation program of Cancer Stem Cells by photo-triggerable Figure 3.4 - Stability of RA+NPs suspended in water or cell culture media. CXCL 4 - chemokine C-X-C motif ligand 4 precursors of B and T lymphocytes13. FGFR - FGF receptor; IL-6, interleukin-6; LSK, lineage−SCA1+KIT+; ROBO4, 

Differential Effects of the Cytokine Thymic Stromal

by CM Cingolani 2013 Although TSLP has been shown to stimulate several immune cells in the TSLP-DCs: Thymic Stromal Lymphopoietin-Primed Dendritic Cells During inflammatory conditions, other cellular precursors of DCs have been found. The high affinity ligand of this receptor, IL-34, might be essential for the.

Maintenance and expansion of hematopoietic stem cells in

by Z Wu Chapter 7 Discussion and Future Work. pre-B-cell leukemia transcription factor 1 maintain or expand HSCs in vitro made use of stromal cell lines that provided a niche 1992); after 4-week culture on the feeder layer, certain clones from 1997). Murine Sca+Lin-. BM. Serum-free,. IL-. 11(100ng/ml),. Flt3L(100ng/ml),.

Progenitor Separation and Assays I Wednesday, June 21

ENRICHMENT OF PERIPHERAL BLOOD STEM CELLS BY cell population contains 83±12%(n=7) of the total CD34+ cells as detected by FACS CD34+/CDw109+ cells cultured for 11 days in recombinant mice and to differentiate into B or NK cells in bone marrow single reagent kit, and 4) pre-selection CD34+ cell.

ADAM10 is a critical regulator of B cell development, antibody

by D Gibb 2010 Effects of B cell-specific ADAM10 deletion and overexpression in This Dissertation is brought to you for free and open access by the Graduate Marginal zone B cell precursor Finally, hematopoietic stem cell cultures revealed that presence of IL-4 (10,000 Units/ml), CD40 ligand trimer (CD40L, 25 ng/ml), and 8:A3.

Techniques for Immune Function Analysis

by BD Biosciences Cited by 9 Chapter 7: ELISA for Specifically Measuring the Levels of Cytokines, of Analytes in Serum, Plasma, and Cell Culture Supernatants. ELISA, BD OptEIA™ ELISA Sets and Kits, Immunofluorescent Staining of (eg, IL-2Rα) by stimulated cells.4 Cytokine receptors transduce external biological populations or cell lines.


by MV Gronda Effect of different cytokines in Stem Pro [email protected] serum-free culture on OCI-LY 17 The addition of IL 6 to both cell lines at initiation of cultures abolished the lag with a surrogate light chain as part d the pre-B cell receptor and permits the cells to known as Mast Cell Growth Factor, Steel Factor or c-kit ligand) has been